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Molecular epidemiology of Staphylococcus aureus from Lambaréné, Gabon

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Abstract

While there is an abundance of data on the epidemiology and molecular typing of Staphylococcus aureus, especially those carrying Panton–Valentine leucocidin (PVL) genes or mecA from Western Europe, Northern America and Australia, comparably few studies target African strains. In this study, we characterised genes associated with virulence and resistance, as well the phylogenetic background of S. aureus from healthy carriers and outpatients in Gabon. In total, 103 isolates from 96 study participants were characterised. Seventy-nine isolates originated from throat swabs and 24 isolates from skin lesions. Three isolates carried mecA, although only one, belonging to CC8-MRSA-IV [PVL+] ‘USA300’, was found to be phenotypically oxacillin-resistant; two CC88-MRSA-IV isolates appeared to be oxacillin-susceptible. PVL genes were common, with a total of 44 isolates (43 %) found to be PVL-positive. CC15-MSSA [PVL+] (n = 29) and CC152-MSSA [PVL+] (n = 9) were the predominant clones among the PVL-positive isolates. Among PVL-negative isolates, CC5-MSSA (n = 12), CC101-MSSA (n = 10) and CC15 (n = 9) were the most frequent. A hitherto undescribed multilocus sequence type of S. schweitzeri was detected twice in unrelated patients. The data emphasise a need for further studies on the role of PVL in African populations and the clinical significance of S. schweitzeri.

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Acknowledgements

The authors acknowledge Dr. H. Hotzel of the Institute of Bacterial Infections and Zoonoses (IBIZ), Friedrich Loeffler Institute (FLI), Jena, for help with performing MLST as well as Prof. E. Jacobs (Dresden) and Dr. R Ehricht (Jena) for their support. We thank the medical and laboratory staff at the CERMEL as well as the participating patients for their cooperation.

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    Authors and Affiliations

    1. Department for Pediatrics, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany

      K. V. Okuda, N. Toepfner, B. Arnold, U. Falke, L. Menschner & R. Berner

    2. Centre de Recherches Médicales de Lambaréné (CERMEL), Lambaréné, Gabon

      A. S. Alabi

    3. Institut für Tropenmedizin, Eberhard Karls Universität Tübingen and Deutsches Zentrum für Infektionsforschung, Tübingen, Germany

      A. S. Alabi & S. Bélard

    4. Department for Pediatric Pneumology and Immunology, Charité-Universitätsmedizin Berlin, Berlin, Germany

      S. Bélard

    5. Institute for Medical Microbiology and Hygiene, “Carl Gustav Carus”, Technische Universität Dresden, Dresden, Germany

      S. Monecke & A. Ruppelt-Lorz

    6. Alere Technologies GmbH/InfectoGnostics Research Campus, Jena, Germany

      S. Monecke

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    The clinical part of this work was supported by the German Society for Paediatric Infectious Diseases (Deutsche Gesellschaft für Pädiatrische Infektiologie), grant number DGPI07/2012. For the molecular studies, there was no external funding. Each of the participating institutions covered the costs of experiments performed in the respective institutions, as well as granted the time needed to perform this study.

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    Stefan Monecke is an employee of Alere Technologies GmbH, Jena, Germany, the company that manufactures the microarrays used for this study. The other authors do not declare conflicts of interest.

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    The study was approved by the CERMEL Institutional Review Board. Informed consent was obtained from all participants prior to any study procedure; for minors, a parent or legal guardian provided written consent.

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    K. V. Okuda, N. Toepfner and Stefan Monecke contributed equally to the study.

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    Okuda, K.V., Toepfner, N., Alabi, A.S. et al. Molecular epidemiology of Staphylococcus aureus from Lambaréné, Gabon. Eur J Clin Microbiol Infect Dis 35, 1963–1973 (2016). https://doi.org/10.1007/s10096-016-2748-z

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