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The MAST® D68C test: an interesting tool for detecting extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae

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Abstract

The Mast® D68C test is a phenotypical test that allows the detection of extended-spectrum β-lactamase (ESBL) production, even in AmpC-producing Enterobacteriaceae. We assessed its detection accuracy against a large collection of 106 Enterobacteriaceae isolates producing a wide diversity of well-characterized β-lactamases (53 ESBL producers, 25 Amp. producers, seven AmpC and ESBL producers, five carbapenemase producers, three carbapenemase and ESBL producers, one AmpC, carbapenemase, and ESBL producer, three TEM-1 producers, three SHV-1 producers, three OXA-1 producers, and one hyperOXY producer, ATCC 35218, ATCC 25922 [a β-lactamase-negative control strain]). The results were compared with those of the double disk test and the Clinical and Laboratory Standards Institute (CLSI) confirmatory test for the detection of ESBL. The sensitivity was 90.6 % for the synergy test, 87.5 % for the CLSI method, and only 73.1 % for D68C, which, however, reached 92.1 % if the strains for which supplementary investigations were recommended and the complex mutant TEM (CMT)-producing strains were excluded versus 94.1 % and 88.2 % for the other methods. The specificity was 90.2 % for the synergy test and 100 % for the CLSI method and D68C. D68C was also efficient in detecting AmpC-overproducing strains (sensitivity = 97 %, specificity = 95.9 %): among the 74 strains belonging to natural AmpC-producing species, the sensitivity and specificity were 100 and 94.8 %, respectively. The Mast® D68C-test is a promising method that is easy to perform for the detection of current ESBLs and could also be useful for the detection of plasmid-encoded AmpC enzymes (sensitivity = 100 %).

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Acknowledgments

We thank Laurent Guillouard, Marlene Jan, and Rolande Perroux for the technical assistance. We thank Mast® Diagnostic for providing the D68C tests. This work was supported in part by a grant from the Ministère de l’Éducation Nationale, Ministère de l’Enseignement Supérieur et de la Recherche, Paris, France.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratoire de Bactériologie, CHU Clermont-Ferrand, 58, rue Montalembert, 63003, Clermont-Ferrand, France

    C. Nourrisson, R. N. Tan, C. Hennequin, L. Gibold, R. Bonnet & F. Robin

  2. Centre National de Référence de la Résistance aux Antibiotiques, Laboratoire Associé “BLSE-Céphalosporinase”, CHU Clermont-Ferrand, Clermont-Ferrand, France

    R. N. Tan, C. Hennequin, R. Bonnet & F. Robin

  3. Clermont-Université, UMR CNRS 6023, Laboratoire Microorganismes: Génome Environnement (LMGE), Université d’Auvergne, Clermont-Ferrand, France

    C. Hennequin

  4. Faculté de Médecine, Clermont-Université, Université d’Auvergne, M2iSH, Clermont-Ferrand, France

    L. Gibold, R. Bonnet & F. Robin

  5. INSERM, U1071, 63001, Clermont-Ferrand, France

    L. Gibold, R. Bonnet & F. Robin

  6. INRA USC2018, 63001, Clermont-Ferrand, France

    L. Gibold, R. Bonnet & F. Robin

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  1. C. Nourrisson
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  2. R. N. Tan
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Correspondence to F. Robin.

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Nourrisson, C., Tan, R.N., Hennequin, C. et al. The MAST® D68C test: an interesting tool for detecting extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. Eur J Clin Microbiol Infect Dis 34, 975–983 (2015). https://doi.org/10.1007/s10096-014-2305-6

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