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Molecular characterization of the extended-spectrum beta-lactamase (ESBL)-producing Shigella spp. in Shanghai

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Abstract

Shigellosis is a public health concern in China. We tested 216 Shigella isolates collected in Shanghai in 2007 for the production of extended-spectrum beta-lactamases (ESBLs). ESBL-producing isolates were characterized using polymerase chain reaction (PCR)-based genotyping, conjugation, pulsed-field gel electrophoresis (PFGE), and DNA sequence analysis of regions adjacent to bla genes. Plasmids containing genes encoding ESBLs were analyzed using plasmid replicon typing. ESBLs were produced by 18.1 % (39/216) of Shigella isolates, and all 39 ESBL-producing strains harbored bla CTX-M genes. CTX-M-14 was the most frequent variant (69.2 %, 27/39), followed by CTX-M-15 (15.4 %, 6/39). All bla CTX-M genes were transferable by conjugation, and the insertion sequence ISEcp1 was detected upstream of all bla CTX-M genes. The CTX-M-producing Shigella isolates showed high clonal diversity. IncI1, IncFII, IncN, and IncB/O replicons were respectively detected in 23 (58.9 %), 9 (23.1 %), 1 (2.6 %), and 1 (2.6 %) of the 39 transconjugants carrying bla CTX-M. The bla CTX-M-14 genes were most frequently carried by IncI1 (n = 13, 48.1 %) or IncFII (n = 9, 33.3 %) plasmids, and the bla CTX-M-15 genes were closely associated with IncI1 (n = 5, 83.3 %). Our findings demonstrate the high prevalence of ESBL-producing Shigella in Shanghai, the importance of plasmids and ISEcp1 as carriers of bla CTX-M genes, and the close association between certain bla CTX-M genes with a specific plasmid.

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Funding

This work was supported by a Shanghai Science and Technology specific project (grant no. 11ZR1422200).

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Authors and Affiliations

  1. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 197 Ruijin 2nd Rd, Shanghai, 200025, China

    J. Li, Y. Ni & J. Sun

  2. Department of Clinical Laboratory, Union Hospital, Fujian Medical University, Fuzhou, Fujian, 350001, China

    B. Li

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  1. J. Li
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  2. B. Li
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  3. Y. Ni
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  4. J. Sun
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Corresponding author

Correspondence to J. Sun.

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Jie Li and Bin Li contributed equally to this work.

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Li, J., Li, B., Ni, Y. et al. Molecular characterization of the extended-spectrum beta-lactamase (ESBL)-producing Shigella spp. in Shanghai. Eur J Clin Microbiol Infect Dis 34, 447–451 (2015). https://doi.org/10.1007/s10096-014-2244-2

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  • DOI: https://doi.org/10.1007/s10096-014-2244-2

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