Abstract
The purpose of this investigation was to compare the performance and cost of the Carba NP test with the Rapid CARB Screen Kit in detecting the presence of carbapenemase in Enterobacteriaceae and Pseudomonas aeruginosa. Ninety-two Enterobacteriaceae and 19 P. aeruginosa strains were used in this study. Multiplex polymerase chain reaction (PCR) was performed to determine whether these microorganisms harboured bla VIM, bla IMP, bla NDM, bla KPC and bla OXA-48. The Carba NP test and Rapid CARB Screen Kit were used on the strains according to the standardised protocols. The sensitivity, specificity and positive and negative predictive values of the tests were calculated. The cost of performing one test was also calculated. Forty-five Enterobacteriaceae and six P. aeruginosa were found to harbour carbapenemase-encoding genes. The Carba NP test had sensitivities of 91.1 % and 100 % for Enterobacteriaceae and P. aeruginosa, respectively. The Rapid CARB Screen Kit had sensitivities of 73.3 % and 66.7 % for Enterobacteriaceae and P. aeruginosa, respectively. The specificity of both tests was 100 %. The approximated price for performing one Carba NP test was 0.31 Euros and for CARB Screen Kit, it was 1.25 Euros. The Carba NP test performed better than the Rapid CARB Screen Kit in detecting carbapenemase production in Enterobacteriaceae and P. aeruginosa. The cost to perform both tests is reasonable.
Similar content being viewed by others
References
Infectious Diseases Society of America (IDSA), Spellberg B, Blaser M et al (2011) Combating antimicrobial resistance: policy recommendations to save lives. Clin Infect Dis 52(Suppl 5):S397–S428
Gupta N, Limbago BM, Patel JB, Kallen AJ (2011) Carbapenem-resistant Enterobacteriaceae: epidemiology and prevention. Clin Infect Dis 53(1):60–67
Nordmann P, Gniadkowski M, Giske CG et al (2012) Identification and screening of carbapenemase-producing Enterobacteriaceae. Clin Microbiol Infect 18(5):432–438
Nordmann P, Poirel L (2013) Strategies for identification of carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother 68(3):487–489
Nordmann P, Poirel L, Dortet L (2012) Rapid detection of carbapenemase-producing Enterobacteriaceae. Emerg Infect Dis 18(9):1503–1507
Huang TD, Poirel L, Bogaerts P, Berhin C, Nordmann P, Glupczynski Y (2014) Temocillin and piperacillin/tazobactam resistance by disc diffusion as antimicrobial surrogate markers for the detection of carbapenemase-producing Enterobacteriaceae in geographical areas with a high prevalence of OXA-48 producers. J Antimicrob Chemother 69(2):445–450
Bogaerts P, Rezende de Castro R, de Mendonça R, Huang TD, Denis O, Glupczynski Y (2013) Validation of carbapenemase and extended-spectrum beta-lactamase multiplex endpoint PCR assays according to ISO 15189. J Antimicrob Chemother 68(7):1576–1582
Tijet N, Boyd D, Patel SN, Mulvey MR, Melano RG (2013) Evaluation of the Carba NP test for rapid detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrob Agents Chemother 57(9):4578–4580
Poirel L, Potron A, Nordmann P (2012) OXA-48-like carbapenemases: the phantom menace. J Antimicrob Chemother 67(7):1597–1606
Dortet L, Poirel L, Errera C, Nordmann P (2014) CarbAcineto NP test for rapid detection of carbapenemase-producing Acinetobacter spp. J Clin Microbiol 52(7):2359–2364
Acknowledgements
We thank Prof. Y. Glupczynski and his collaborators at the National Reference Center for ESBL and CPE, and antibiotic-resistant Pseudomonas and Acinetobacter, for the confirmation and characterization of the carbapenemases produced by the strains used in this study. We also thank Prof. P. Nordmann from the University of Fribourg, Switzerland for his comments on the manuscript.
Conflict of interest
The authors declare that they have no conflict of interest.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yusuf, E., Van Der Meeren, S., Schallier, A. et al. Comparison of the Carba NP test with the Rapid CARB Screen Kit for the detection of carbapenemase-producing Enterobacteriaceae and Pseudomonas aeruginosa . Eur J Clin Microbiol Infect Dis 33, 2237–2240 (2014). https://doi.org/10.1007/s10096-014-2199-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10096-014-2199-3