European Journal of Clinical Microbiology & Infectious Diseases

, Volume 33, Issue 7, pp 1223–1228

Genetic diversity of OXA-51-like genes among multidrug-resistant Acinetobacter baumannii in Riyadh, Saudi Arabia

Authors

  • M. Aly
    • King Abdullah International Medical Research Centre
  • H. T. Tayeb
    • King Faisal Specialist Hospital & Research Centre
  • S. M. Al Johani
    • King Saud bin Abdulaziz University for Health Sciences, WHO CC and GCC Center for Infection Control
    • Department of PathologyKing Abdulaziz Medical City
  • E. J. Alyamani
    • King Abdulaziz City for Science and Technology
  • F. Aldughaishem
    • Department of PathologyKing Abdulaziz Medical City
  • I. Alabdulkarim
    • King Abdullah International Medical Research Centre
    • King Abdullah International Medical Research Centre
    • King Saud bin Abdulaziz University for Health Sciences, WHO CC and GCC Center for Infection Control
    • Department of Infection Prevention and ControlKing Abdulaziz Medical City
Article

DOI: 10.1007/s10096-014-2068-0

Cite this article as:
Aly, M., Tayeb, H.T., Al Johani, S.M. et al. Eur J Clin Microbiol Infect Dis (2014) 33: 1223. doi:10.1007/s10096-014-2068-0

Abstract

We explore the genetic diversity of class D oxacillinases, including OXA-23, -24 (-40), -58 and, particularly, the intrinsic OXA-51-like genes, among multidrug-resistant (MDR) Acinetobacter baumannii strains from inpatients at a tertiary care hospital in Riyadh, Saudi Arabia. Sequence-based typing (SBT) of the OXA-51-like gene was carried out on 253 isolates. Selected isolates (n = 66) were subjected to multilocus sequence typing (MLST). The polymerase chain reaction (PCR) typing results showed that all isolates (n = 253) contained the OXA-51-like and OXA-23 genes. However, the OXA-58 gene was detected in five isolates. Further, none of the isolates had the OXA-40 (identical to the OXA-24) gene. SBT revealed a high OXA-51-like genotypic diversity and showed that all isolates were clustered into four main groups: OXA-66 (62.3 %), followed by OXA-69 (19.1 %), OXA-132 (7.6 %) and other OXA-51-like genes (10.3 %), including OXA-79, -82, -92, -131 and -197. MLST revealed four main sequence types (STs), 2, 19, 20 and 25, among the isolates, in addition to six isolates with newly designated ST194–ST197 singletons. Further, a high prevalence (81.4 %) of OXA-66 and OXA-69-like genes in A. baumannii was identified. More studies are essential in order to explore the molecular mechanisms that confer carbapenem-resistant phenotypes for A. baumannii isolates and to investigate the genetic diversity of other OXA-D genes.

Copyright information

© Springer-Verlag Berlin Heidelberg 2014