Article

European Journal of Clinical Microbiology & Infectious Diseases

, Volume 33, Issue 7, pp 1223-1228

First online:

Genetic diversity of OXA-51-like genes among multidrug-resistant Acinetobacter baumannii in Riyadh, Saudi Arabia

  • M. AlyAffiliated withKing Abdullah International Medical Research Centre
  • , H. T. TayebAffiliated withKing Faisal Specialist Hospital & Research Centre
  • , S. M. Al JohaniAffiliated withKing Saud bin Abdulaziz University for Health Sciences, WHO CC and GCC Center for Infection ControlDepartment of Pathology, King Abdulaziz Medical City
  • , E. J. AlyamaniAffiliated withKing Abdulaziz City for Science and Technology
  • , F. AldughaishemAffiliated withDepartment of Pathology, King Abdulaziz Medical City
  • , I. AlabdulkarimAffiliated withKing Abdullah International Medical Research Centre
  • , H. H. BalkhyAffiliated withKing Abdullah International Medical Research CentreKing Saud bin Abdulaziz University for Health Sciences, WHO CC and GCC Center for Infection ControlDepartment of Infection Prevention and Control, King Abdulaziz Medical City Email author 

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Abstract

We explore the genetic diversity of class D oxacillinases, including OXA-23, -24 (-40), -58 and, particularly, the intrinsic OXA-51-like genes, among multidrug-resistant (MDR) Acinetobacter baumannii strains from inpatients at a tertiary care hospital in Riyadh, Saudi Arabia. Sequence-based typing (SBT) of the OXA-51-like gene was carried out on 253 isolates. Selected isolates (n = 66) were subjected to multilocus sequence typing (MLST). The polymerase chain reaction (PCR) typing results showed that all isolates (n = 253) contained the OXA-51-like and OXA-23 genes. However, the OXA-58 gene was detected in five isolates. Further, none of the isolates had the OXA-40 (identical to the OXA-24) gene. SBT revealed a high OXA-51-like genotypic diversity and showed that all isolates were clustered into four main groups: OXA-66 (62.3 %), followed by OXA-69 (19.1 %), OXA-132 (7.6 %) and other OXA-51-like genes (10.3 %), including OXA-79, -82, -92, -131 and -197. MLST revealed four main sequence types (STs), 2, 19, 20 and 25, among the isolates, in addition to six isolates with newly designated ST194–ST197 singletons. Further, a high prevalence (81.4 %) of OXA-66 and OXA-69-like genes in A. baumannii was identified. More studies are essential in order to explore the molecular mechanisms that confer carbapenem-resistant phenotypes for A. baumannii isolates and to investigate the genetic diversity of other OXA-D genes.