European Journal of Clinical Microbiology & Infectious Diseases

, Volume 31, Issue 10, pp 2783–2790

Molecular epidemiology of the Bacillus anthracis isolates collected throughout Turkey from 1983 to 2011

  • R. Durmaz
  • M. Doganay
  • M. Sahin
  • D. Percin
  • M. K. Karahocagil
  • U. Kayabas
  • B. Otlu
  • A. Karagoz
  • F. Buyuk
  • O. Celebi
  • Z. Ozturk
  • M. Ertek
  • Anthrax Study Group
Article

DOI: 10.1007/s10096-012-1628-4

Cite this article as:
Durmaz, R., Doganay, M., Sahin, M. et al. Eur J Clin Microbiol Infect Dis (2012) 31: 2783. doi:10.1007/s10096-012-1628-4

Abstract

The main perspective of this study was to determine cross-transmissions amongst anthrax cases and provide detailed information regarding the genotypes of Bacillus anthracis isolates circulating in Turkey. A total of 251 B. anthracis isolates were obtained from human (93 isolates), animal (155 isolates), and environmental (three isolates) samples in various provinces of Turkey. All isolates were susceptible to quinolones, vancomycin, tigecycline, and linezolid, but not to ceftriaxone. Excluding human isolates, one of the animal isolates was found to be resistant to penicillin, erythromycin, and doxycycline. Multiple-locus variable-number tandem repeats analysis including 8 loci (MLVA8) revealed 12 genotypes, in which genotype 43 was observed at the highest frequency (41.8 %), followed by genotype 35 (25.5 %) and genotype 27 (10.4 %). Major subtype A3.a was the predominant cluster, including 86.8 % of the isolates. The MLVA25 analysis for the 251 isolates yielded 62 different genotypes, 33 of which had only one isolate, while the remaining 29 genotypes had 2 to 43 isolates, with a total of 218 isolates (86.9 %). These findings indicate very high cross-transmission rates within anthrax cases in Turkey. The genotypes diagnosed in Turkey are populated in the A major cluster. Penicillin prescribed as the first-choice antibiotic for the treatment of anthrax is still effective.

Supplementary material

10096_2012_1628_MOESM1_ESM.docx (144 kb)
Additional File 1This file contains a dendrogram of the 62 different MLVA25 genotypes of the 251 Bacillus anthracis isolates from Turkey. (DOCX 144 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • R. Durmaz
    • 1
    • 2
  • M. Doganay
    • 3
  • M. Sahin
    • 4
  • D. Percin
    • 5
  • M. K. Karahocagil
    • 6
  • U. Kayabas
    • 7
  • B. Otlu
    • 8
  • A. Karagoz
    • 2
  • F. Buyuk
    • 4
  • O. Celebi
    • 4
  • Z. Ozturk
    • 9
  • M. Ertek
    • 10
  • Anthrax Study Group
  1. 1.Department of Clinical Microbiology, Medical FacultyKirikkale UniversityKirikkaleTurkey
  2. 2.Molecular Microbiology Research and Application LaboratoryRefik Saydam National Public Health AgencyAnkaraTurkey
  3. 3.Department of Infectious Disease, Medical FacultyErciyes UniversityKayseriTurkey
  4. 4.Department of Microbiology, Veterinary FacultyKafkas UniversityKarsTurkey
  5. 5.Department of Clinical Microbiology, Medical FacultyErciyes UniversityKayseriTurkey
  6. 6.Department of Infectious Disease, Medical Faculty100 Yil UniversityVanTurkey
  7. 7.Department of Infectious Disease, Medical FacultyInonu UniversityMalatyaTurkey
  8. 8.Department of Clinical Microbiology, Medical FacultyInonu UniversityMalatyaTurkey
  9. 9.Department of Infectious Disease, Medical FacultyAtatürk UniversityErzurumTurkey
  10. 10.Refik Saydam National Public Health AgencyAnkaraTurkey