Skip to main content
Log in

Analysis of microbiomes in three traditional starters and volatile components of the Chinese rice wines

  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

To understand the effect of microbial community on the flavor of fermented rice wine, microbiomes in three traditional starters (CMQ, NBQ, and YCQ) from different origins for making Chinese rice wines were evaluated and the volatile components of their rice wines were compared. The results showed that the dominant genera in CMQ were Pantoea, Bacillus, Rhizopus, and Candida, the dominant microorganisms in NBQ were Pediococcus, Lactobacillus, Acetobacter, Weissella, Bacillus, Rhizopus, Candida, and Aspergillus, the dominant microorganisms in YCQ were Pediococcus, Lactobacillus, Leuconostoc, Weissella, Lactococcus, Ochrobactrum, Rhizopus, and Mucor. There were significant differences in sensory properties of the wines brewed by three starters. Although the major aroma components were benzyl alcohol, 2-octanone, benzoic acid, and phenethyl acetate, each rice wine had its own main aroma components include 1-octanol, 1-pentanol, ethyl acetate, etc. The results showed that the different microbial communities in starter results in the significant difference of the aroma components in its fermented rice wine.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Barbara B, George S, Ashok P, Sabu A, James CL, Robert PT. Production of phytase by Mucor racemosus in solid-state fermentation. Biotechnol. Prog. 19: 312-319 (2010)

    Google Scholar 

  • Cai H, Zhang T, Zhang Q, Luo J, Cai C, Mao J. Microbial diversity and chemical analysis of the starters used in traditional Chinese sweet rice wine. Food Microbiol. 73: 319-326 (2018)

    Article  CAS  Google Scholar 

  • Caporaso JG., Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI, Huttley GA, Kelley ST, Knights D, Koenig JE, Ley RE, Lozupone CA, McDonald D, Muegge BD, Pirrung M, Reeder J, Sevinsky JR, Turnbaugh PJ, Walters WA, Widmann J, Yatsunenko T, Zaneveld J, Knight R. QIIME allows analysis of high-throughput community sequencing data. Nat. Methods 7: 335-336 (2010)

    Article  CAS  Google Scholar 

  • Chen S, Xu Y. Adaptive evolution of Saccharomyces cerevisiae with enhanced ethanol tolerance for Chinese rice wine fermentation. Appl. Biochem. Biotechnol. 173: 1940-1954 (2014)

    Article  CAS  Google Scholar 

  • Chu L, Zan X, Tang X, Zhao L, Chen H, Chen YQ, Chen W, Song Y. The role of a xylose isomerase pathway in the conversion of xylose to lipid in Mucor circinelloides. RSC Adv. 6: 77944-77952 (2016)

    Article  CAS  Google Scholar 

  • Ercolini D. High-throughput sequencing and metagenomics: moving forward in the culture-independent analysis of food microbial ecology. Appl. Environ. Microb. 79: 3148-3155 (2013)

    Article  CAS  Google Scholar 

  • Huang Z, Guo W, Zhou W, Li L, Xu J, Hong J, Liu H, Zeng F, Bai W, Liu B, Ni L, Rao P, Lv X. Microbial communities and volatile metabolites in different traditional fermentation starters used for Hong Qu glutinous rice wine. Food Res. Int. 121: 593-603 (2018a)

    Article  Google Scholar 

  • Huang ZR, Hong JL, Xu JX, Li L, Guo WL, Pan YY , Chen SJ, Bai WD, Rao PF, Ni L, Zhao LN, Liu B, Lv XC. Exploring core functional microbiota responsible for the production of volatile flavour during the traditional brewing of Wuyi Hong Qu glutinous rice wine. Food Microbiol. 76: 487-496 (2018b)

    Article  CAS  Google Scholar 

  • Jin GY, Zhu Y, Xu Y. Mystery behind Chinese liquor fermentation. Trends Food Sci. Tech. 63: 18-28 (2017)

    Article  CAS  Google Scholar 

  • Kong Y, Wu Q, Zhang Y, Xu Y. In situ analysis of metabolic characteristics reveals the key yeast in the spontaneous and solid-state fermentation process of Chinese light-style liquor. Appl. Environ. Microbiol. 80: 3667-3376 (2014)

    Article  Google Scholar 

  • Langille MGI, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, Clemente JC, Burkepile DE, Thurber RLV, Knight R, Beiko R, Huttenhower C. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat. Biotechnol. 31: 814-821 (2013)

    Article  CAS  Google Scholar 

  • Lemos LN, Fulthorpe RR, Triplett EW, Roesch LF. Rethinking microbial diversity analysis in the high throughput sequencing era. J. Microbiol. Methods 86: 42-51 (2011)

    Article  CAS  Google Scholar 

  • Li Z, Bai Z, Wang D, Zhang W. Cultivable bacterial diversity and amylase production in three typical Daqus of Chinese spirits. Int. J. Food Sci. Tech. 49: 776-786 (2014)

    Article  Google Scholar 

  • Liu SP, Mao J, Liu YY, Meng XY, Ji ZW, Zhou ZL, Aisikaer A. Bacterial succession and the dynamics of volatile compounds during the fermentation of Chinese rice wine from Shaoxing region. World J. Microbiol. Biotechnol. 31: 1907-1921 (2015)

    Article  CAS  Google Scholar 

  • Liu SP, Yu JX, Wei XL, Ji ZW, Zhou ZL, Meng XY, Mao J. Sequencing-based screening of functional microorganism to decrease the formation of biogenic amines in Chinese rice wine. Food Control. 64: 98-104 (2016)

    Article  CAS  Google Scholar 

  • Maayer PD, Aliyu H, Vikram S, Blom J, Duffy B, Cowan DA, Smits THM, Venter SN, Coutinho TA. Phylogenomic, pangenomic, pathogenomic and evolutionary genomic insights into the agronomically relevant entero bacteria Pantoea ananatis and Pantoea stewartii. Front. Microbiol. 8: 1755-1768 (2017)

    Article  Google Scholar 

  • Meng X, Wu Q, Wang L, Wang D, Chen L, Xu Y. Improving flavor metabolism of Saccharomyces cerevisiae by mixed culture with Bacillus licheniformis for Chinese Maotai-flavor liquor making. J. Ind. Microbiol. Biotechnol. 42: 1601-1608 (2015)

    Article  CAS  Google Scholar 

  • Petya V, Kaloyan P, Valentin L, Flora T, Anton S, Zhengjun W. Microbial diversity and health-promoting properties of the traditional bulgarian yogurt. Biotechnol. Biotechnol. Equip. 32: 1-13 (2018)

    Article  Google Scholar 

  • Ponnusamy K, Lee S, Lee CH. Time-dependent correlation of the microbial community and the metabolomics of traditional barley nuruk starter fermentation. Biosci. Biotechnol. Biochem. 77: 683-690 (2013)

    Article  Google Scholar 

  • Song SH, Lee C, Lee S, Park JM, Lee HJ, Bai DH, Yoon SS, Choi JB, Park YS. Analysis of microflora profile in Korean Traditional Nuruk. J. Microbiol. Biotechnol. 23: 40-46 (2013)

    Article  CAS  Google Scholar 

  • Stellato G, Storia AL, Filippis FD, Borriello G, Villani F, Ercolini D. Overlap of spoilage-associated microbiota between meat and the meat processing environment in small-scale and large-scale retail distributions. Appl. Environ. Microbiol. 82: 4045-4054 (2016)

    Article  CAS  Google Scholar 

  • Tao Y, Li J, Rui J, Xu Z, Zhou Y, Hu X, Wang X, Liu M, Li D, Li X. Prokaryotic communities in pit mud from different-aged cellars used for the production of Chinese strong-flavored liquor. Appl. Environ. Microb. 80: 2254-2260 (2014)

    Article  Google Scholar 

  • Wang P, Wu Q, Jiang X, Wang Z, Tang J, Xua Y. Bacillus licheniformis, affects the microbial community and metabolic profile in the spontaneous fermentation of Daqu starter for Chinese liquor making. Int. J. Food Microbiol. 250: 59-67 (2017)

    Article  CAS  Google Scholar 

  • Wang Z, Shao Y. Effects of microbial diversity on nitrite concentration in pao cai, a naturally fermented cabbage product from China. Food Microbiol. 72: 185-192 (2018)

    Article  CAS  Google Scholar 

  • Welke JE, Zanus M, Lazzarotto M, Zinid CA. Quantitative analysis of headspace volatile compounds using comprehensive two-dimensional gas chromatography and their contribution to the aroma of Chardonnay wine. Food Res. Int. 59: 85-99 (2014)

    Article  CAS  Google Scholar 

  • Wu Q, Zhu W, Wang W, Xu Y. Effect of yeast species on the terpenoids profile of Chinese light-style liquor. Food Chem. 168: 390-395 (2015)

    Article  CAS  Google Scholar 

  • Yang Y, Xia Y, Wang G, Yu J, Ai L. Effect of mixed yeast starter on volatile flavor compounds in Chinese rice wine during different brewing stages. Lwt-Food Sci. Technol. 78: 373-381 (2017a)

    Article  CAS  Google Scholar 

  • Yang Y, Xia Y, Wang G, Zhang H, Xiong Z, Yu J, Yu H, Ai L. Comparison of oenological property, volatile profile, and sensory characteristic of Chinese rice wine fermented by different starters during brewing. Int. J. Food Prop. 20: 1-17 (2017b)

    Article  Google Scholar 

  • Yu H Y, Zhao J, Li F, Tian H, Ma X. Characterization of Chinese rice wine taste attributes using liquid chromatographic analysis, sensory evaluation, and an electronic tongue. J. Chromatogr. B 997: 129-135 (2015)

    Article  CAS  Google Scholar 

  • Yu L, Ding F, Ye H. Analysis of characteristic flavour compounds in Chinese rice wines and representative fungi in wheat Qu samples from different regions. J. Inst. Brew. 118: 114-119 (2012)

    Article  CAS  Google Scholar 

  • Zheng XW, Tabrizi MR, Nout M, Han BZ. Daqu—a traditional Chinese liquor fermentation starter. J. I. Brewing. 117: 82-90 (2011)

    Article  CAS  Google Scholar 

  • Zheng XW, Yan Z, Han BZ, Zwietering MH, Samson RA, Boekhout T, Robert Nout MJ. Complex microbiota of a Chinese Fen liquor fermentation starter (Fen-Daqu), revealed by culture-dependent and culture-independent methods. Food Microbiol. 31: 293-300 (2012)

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by Science and Technology Commission of Shanghai Municipality (CN) (No. 18495810900).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Xia Ma.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Human and animal rights

This article does not contain any studies with human or animal subjects performed by the any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chen, L., Ren, L., Li, D. et al. Analysis of microbiomes in three traditional starters and volatile components of the Chinese rice wines. Food Sci Biotechnol 30, 87–96 (2021). https://doi.org/10.1007/s10068-020-00839-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-020-00839-y

Keywords

Navigation