Abstract
This study compared the antioxidant activity of extracts from Dendropanax morbifera (D. morbifera) Levillis leaves. The concentrations of flavonoids and polyphenols were measured in extracts of D. morbifera leaves. The antioxidant activities were examined by ABTS and DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP). Total flavonoid and polyphenol contents, and FRAP were highest in the 30% ethanol extract collected in May. The ABTS and DPPH radical scavenging activities were the highest in the 60% ethanol extract harvested in May. For investigating the relationship between antioxidant activity and specific polyphenols, rutin and chlorogenic acid of the polyphenol component were quantified by LC–MS/MS analysis. The concentrations of them were highest in the 60% ethanol extract collected in May, and showed positive correlations with antioxidant activities. The optimal extraction conditions to yield the most effective antioxidant activity were obtained using a 60% ethanol extraction solvent with samples collected in May.
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Kornfeld OS, Hwang S, Disatnik MH, Chen CH, Qvit N, Mochly-Rosen D (2015) Mitochondrial reactive oxygen species at the heart of the matter: new therapeutic approaches for cardiovascular diseases. Circ Res 116:1783–1799
Cho M, Ko SB, Kim JM, Lee OH, Lee DW, Kim JY (2016) Influence of extraction conditions on antioxidant activities and catechin content from bark of Ulmus pumila L. Appl Biol Chem 59:329–336
Moniczewski A, Gawlik M, Smaga I, Niedzielska E, Krzek J, Przegalinski E, Pera J, Filip M (2015) Oxidative stress as an etiological factor and a potential treatment target of psychiatric disorders. Part 1. Chemical aspects and biological sources of oxidative stress in the brain. Pharmacol Rep 67:560–568
Hanus J, Zhang H, Wang Z, Liu Q, Zhou Q, Wang S (2013) Induction of necrotic cell death by oxidative stress in retinal pigment epithelial cells. Cell Death Dis 4:e965
Sinha N, Dabla PK (2015) Oxidative stress and antioxidants in hypertension–a current review. Curr Hypertens Rev 11:132–142
Perez-Cornago A, Lopez-Legarrea P, de la Iglesia R, Lahortiga F, Martinez JA, Zulet MA (2014) Longitudinal relationship of diet and oxidative stress with depressive symptoms in patients with metabolic syndrome after following a weight loss treatment: the RESMENA project. Clin Nutr 33:1061–1067
Kirkham PA, Barnes PJ (2013) Oxidative stress in COPD. Chest 144:266–273
Hamishehkar H, Khani S, Kashanian S, Ezzati Nazhad Dolatabadi J, Eskandani M (2014) Geno- and cytotoxicity of propyl gallate food additive. Drug Chem Toxicol 37:241–246
Taghvaei M, Jafari SM (2015) Application and stability of natural antioxidants in edible oils in order to substitute synthetic additives. J Food Sci Technol 52:1272–1282
Park BY, Min BS, Oh SR, Kim JH, Kim TJ, Kim DH, Bae KH, Lee HK (2004) Isolation and anticomplement activity of compounds from Dendropanax morbifera. J Ethnopharmacol 90:403–408
Kim LS, Youn SH, Kim JY (2014) Comparative Study on Antioxidant Effects of Extracts from Rubus coreanus and Rubus occidentalis. J Korean Soc Food Sci Nutr 43:1357–1362
Kim W, Yim HS, Yoo DY, Jung HY, Kim JW, Choi JH, Yoon YS, Kim DW, Hwang IK (2016) Dendropanax morbifera Leveille extract ameliorates cadmium-induced impairment in memory and hippocampal neurogenesis in rats. BMC Complement Altern Med 16:452
Hyun TK, Kim MO, Lee H, Kim Y, Kim E, Kim JS (2013) Evaluation of anti-oxidant and anti-cancer properties of Dendropanax morbifera Leveille. Food Chem 141:1947–1955
Chung IM, Song HK, Kim SJ, Moon HI (2011) Anticomplement activity of polyacetylenes from leaves of Dendropanax morbifera Leveille. Phytother Res 25:784–786
Moon HI (2011) Antidiabetic effects of dendropanoxide from leaves of Dendropanax morbifera Leveille in normal and streptozotocin-induced diabetic rats. Hum Exp Toxicol 30:870–875
Nakamura M, Ra JH, Kim JS (2016) The comparative analysis of antioxidant and biological activity for the Dendropanax morbifera LEV. Leaves extracted by different ethanol concentrations. Yakugaku Zasshi 136:1285–1296
Ahn JC, Kim SH, Kim MY, Kim OT, Kim KS, Hwang B (2003) Seasonal variations in yields of Hwangchil lacquer and major sesquiterpene compounds from selected superior individuals of Dendropanax morbifera Lev. J Plant Biol 46:38–40
Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG (2005) Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem 91:571–577
Kim W, Kim DW, Yoo DY, Jung HY, Nam SM, Kim JW, Hong SM, Kim DW, Choi JH, Moon SM, Yoon YS, Hwang IK (2014) Dendropanax morbifera Leveille extract facilitates cadmium excretion and prevents oxidative damage in the hippocampus by increasing antioxidant levels in cadmium-exposed rats. BMC Complement Altern Med 14:428
Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237
Mensor LL, Menezes FS, Leitão GG, Reis AS, Dos Santos TC, Coube CS, Leitão SG (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 15:127–130
Dudonne S, Vitrac X, Coutiere P, Woillez M, Merillon JM (2009) Comparative study of antioxidant properties and total phenolic content of 30 plant extracts of industrial interest using DPPH, ABTS, FRAP, SOD, and ORAC assays. J Agric Food Chem 57:1768–1774
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochem 239:70–76
Reisz JA, Wither MJ, Dzieciatkowska M, Nemkov T, Issaian A, Yoshida T, Dunham AJ, Hill RC, Hansen KC, D’Alessandro A (2016) Oxidative modifications of glyceraldehyde 3-phosphate dehydrogenase regulate metabolic reprogramming of stored red blood cells. Blood 128:e32–42
Rampler E, Coman C, Hermann G, Sickmann A, Ahrends R, Koellensperger G (2017) LILY-lipidome isotope labeling of yeast: in vivo synthesis of 13C labeled reference lipids for quantification by mass spectrometry. Analyst 142:1891–1899
Kim YJ, Shin Y (2015) Antioxidant profile, antioxidant activity, and physicochemical characteristics of strawberries from different cultivars and harvest locations. J Korean Soc Appl Bi 58:587–595
Yang H, Shin Y (2017) Antioxidant compounds and activities of edible roses (Rosa hybrida spp.) from different cultivars grown in Korea. Appl Biol Chem 60:129–136
Kim KY, Park MH, Kim KH, Im SH, Park YH, Kim YN (2009) Analysis of chemical composition and in vitro anti-oxidant properties of extracts from sea buckthorn (Hippophae rhamnoides). J Appl Biol Chem 52:58–64
Rahman MM, Islam MB, Biswas M, Khurshid Alam AH (2015) In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Res Notes 8:621
Tai A, Ohno A, Ito H (2016) Isolation and Characterization of the 2,2′-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) Radical Cation-Scavenging Reaction Products of Arbutin. J Agric Food Chem 64:7285–7290
Chen TS, Liou SY, Wu HC, Tsai FJ, Tsai CH, Huang CY, Chang YL (2010) New analytical method for investigating the antioxidant power of food extracts on the basis of their electron-donating ability: comparison to the ferric reducing/antioxidant power (FRAP) assay. J Agric Food Chem 58:8477–8480
Azevedo MI, Pereira AF, Nogueira RB, Rolim FE, Brito GA, Wong DV, Lima-Júnior RC, de Albuquerque Ribeiro R, Vale ML (2013) The antioxidant effects of the flavonoids rutin and quercetin inhibit oxaliplatin-induced chronic painful peripheral neuropathy. Mol Pain 9:53
Sato Y, Itagaki S, Kurokawa T, Ogura J, Kobayashi M, Hirano T, Sugawara M, Iseki K (2011) In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int J Pharm 403:136–138
Hu W, Guo T, Jiang WJ, Dong GL, Chen DW, Yang SL, Li HR (2015) Effects of ultrahigh pressure extraction on yield and antioxidant activity of chlorogenic acid and cynaroside extracted from flower buds of Lonicera japonica. Chin J Nat Med 13:445–453
Kareem HS, Ariffin A, Nordin N, Heidelberg T, Abdul-Aziz A, Kong KW, Yehye WA (2015) Correlation of antioxidant activities with theoretical studies for new hydrazone compounds bearing a 3,4,5-trimethoxy benzyl moiety. Eur J Med Chem 103:497–505
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The results have been undertaken with the support from the Agency for Jeju Plant Resources Dendropanax.
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Youn, J.S., Kim, YJ., Na, H.J. et al. Antioxidant activity and contents of leaf extracts obtained from Dendropanax morbifera LEV are dependent on the collecting season and extraction conditions. Food Sci Biotechnol 28, 201–207 (2019). https://doi.org/10.1007/s10068-018-0352-y
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DOI: https://doi.org/10.1007/s10068-018-0352-y