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Compositional analysis of walnut lipid extracts and properties as an anti-cancer stem cell regulator via suppression of the self-renewal capacity

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Abstract

Colon cancer is a leading cause of cancer-related deaths worldwide. Effects of walnut (Juglans regia L.) lipid extracts (WLEs) on the self-renewal capacity of cancer stem cells (CSCs) in colon cancer were investigated. The dominant component of WLEs was α-linoleic acid (64.6%), followed by α-linolenic acid (14.6%), and oleic acid (12.6%). A higher concentration of γ-tocopherol (37.1%) was also present than of α-tocopherol (0.6%). CD133+CD44+CSCs treated with WLEs showed inhibition of colony formation and sphere formation, indicating a decrease in the self-renewal capacity. Treatment with WLEs also resulted in down-regulation of protein levels, including Notch1, phospho-GSK3β (p-GSK3β), and β-catenin, which are associated with CSCs and the self-renewing capacity. WLEs rich in essential fatty acids and γ-tocopherol can exert therapeutic actions on colon cancer via targeting of CSCs.

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Authors and Affiliations

  1. Department of Nutritional Science and Food Management, Ewha Womans University, Seoul, 03760, Korea

    Jooyeon Chung, Yoo-Sun Kim, Jisoo Lee & Yuri Kim

  2. Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, 16419, Korea

    Jae Hwan Lee

  3. Chaum Life Center, CHA University, Seoul, 06062, Korea

    Sang-Woon Choi

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  1. Jooyeon Chung
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  2. Yoo-Sun Kim
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  3. Jisoo Lee
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  4. Jae Hwan Lee
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  6. Yuri Kim
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Correspondence to Yuri Kim.

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Chung, J., Kim, YS., Lee, J. et al. Compositional analysis of walnut lipid extracts and properties as an anti-cancer stem cell regulator via suppression of the self-renewal capacity. Food Sci Biotechnol 25, 623–629 (2016). https://doi.org/10.1007/s10068-016-0087-6

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  • DOI: https://doi.org/10.1007/s10068-016-0087-6

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