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Japanese bog orchid (Eupatorium japonicum) extract suppresses expression of inducible nitric oxide synthase and cyclooxygenase-2 induced by toll-like receptor agonists

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Abstract

Toll-like receptors (TLR) play an important role in the recognition of many pathogen-associated molecular patterns and the induction of innate immunity. Dysregulated activation of TLR signaling pathways is associated with certain inflammatory diseases. Japanese bog orchid (Eupatorium japonicum), which belongs to a family of Asteraceae plants, is consumed as a tea. The present study investigated the effect of the ethanol extracts of flowers of Japanese bog orchid (EJE) on nuclear factor (NF)-κB activation and expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) by TLR agonists in murine macrophages. EJE suppressed NF-κB activation and iNOS and COX-2 expression induced by lipopolysaccharide (TLR4 agonist), polyriboinosinic polyribocytidylic acid (TLR3), and macrophage-activating 2 kDa lipopeptide (TLR2 and TLR6). These results suggest that EJE can regulate TLR signaling pathways and indicated its potential as a potent anti-inflammatory drug.

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Authors and Affiliations

  1. Departments of Medical Science, College of Medical Sciences, SoonChunHyang University, Asan, Chungnam, 336-745, Korea

    Gyo-Jeong Gu, Hwa-Jeong Shin & Hyung-Sun Youn

  2. Department of Biomedical Laboratory Science, College of Medical Sciences, SoonChunHyang University, Asan, Chungnam, 336-745, Korea

    Sang-Hoon Eom & Hyung-Sun Youn

  3. Department of Food Science and Nutrition, Hallym University, Chuncheon, Gangwon, 200-702, Korea

    Ji Hun Paek & Soon Sung Lim

  4. Department of Biological Environment, Kangwon National University, Chuncheon, Gangwon, 200-701, Korea

    Songmun Kim

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  1. Gyo-Jeong Gu
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  2. Sang-Hoon Eom
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  4. Ji Hun Paek
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  5. Songmun Kim
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  7. Hyung-Sun Youn
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Correspondence to Hyung-Sun Youn.

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Gu, GJ., Eom, SH., Shin, HJ. et al. Japanese bog orchid (Eupatorium japonicum) extract suppresses expression of inducible nitric oxide synthase and cyclooxygenase-2 induced by toll-like receptor agonists. Food Sci Biotechnol 22, 811–815 (2013). https://doi.org/10.1007/s10068-013-0149-y

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  • DOI: https://doi.org/10.1007/s10068-013-0149-y

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