Skip to main content
Log in

Induction of apoptosis by Myagropsis myagroides extracts is associated with a caspase dependent pathway and reactive oxygen species generation in human cancer cells

  • Research Article
  • Published:
Food Science and Biotechnology Aims and scope Submit manuscript

Abstract

The purpose of this study was to elucidate the mechanisms underlying apoptosis induced by an ethanol extracts from Myagropsis myagroides (ME) in HeLa, U937, and PC-3 cells. ME treatment for 24 h significantly inhibited cell viability in a dose-dependent manner, and induced apoptosis. Moreover, ME treatment triggered the cleavage of caspase-8, −9, −3, and poly(ADP-ribose) polymerase (PARP). A general caspase inhibitor (z-VAD-fmk) inhibited ME-induced activation of caspase-3, PARP cleavage, and cell death. ME treatment also triggered the release of cytochrome c from the mitochondria to the cytosol and stimulated the cleavage of Bid, up-regulation of Bax, and down-regulation of Bcl-2. Furthermore, ME treatment caused reactive oxygen species (ROS) generation. An antioxidant N-acetylcysteine (NAC) blocked MEinduced activation of caspase-3, PARP cleavage, and cell death. Overall, these results suggest that ME-induced apoptosis is mediated by a caspase dependent pathway and ROS generation in HeLa, U937, and PC-3 cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Fulda S. Caspase-8 in cancer biology and therapy. Cancer Lett. 281: 128–133 (2009)

    Article  CAS  Google Scholar 

  2. Mellier G, Huang S, Shenoy K, Pervaiz S. TRAILing death in cancer. Mol. Aspects Med. 31: 93–112 (2010)

    Article  CAS  Google Scholar 

  3. Tan ML, Ooi JP, Ismail N, Moad AIH, Muhammad TST. Programmed cell death pathways and current antitumor targets. Pharm. Res. 26: 1547–1560 (2009)

    Article  CAS  Google Scholar 

  4. Wu GS. TRAIL as a target in anti-cancer therapy. Cancer Lett. 285: 1–5 (2009)

    Article  CAS  Google Scholar 

  5. Cao XH, Wang AH, Wang CL, Mao DZ, Lu MF, Cui YQ, Jiao RZ. Surfactin induces apoptosis in human breast cancer MCF-7 cells through a ROS/JNK-mediated mitochondrial/caspase pathway. Chem. Biol. Interact. 183: 357–362 (2010)

    Article  CAS  Google Scholar 

  6. Li-Weber M. Targeting apoptosis pathways in cancer by Chinese medicine. Cancer Lett. doi:10.1016/j.canlet.2010.07.015 (2010)

  7. Jemal A, Siegel R, Xu J, Ward E. Cancer Statistics, 2010. CA-Cancer J. Clin. 60: 277–300 (2010)

    Article  Google Scholar 

  8. Nobili S, Lippi D, Witort E, Donnini M, Bausi L, Mini E, Capaccioli S. Natural compounds for cancer treatment and prevention. Pharmacol. Res. 59: 365–378 (2009)

    Article  CAS  Google Scholar 

  9. Athukorala Y, Lee KW, Kim SK, Jeon YJ. Anticoagulant activity of marine green and brown algae collected from Jeju Island in Korea. Bioresource Technol. 98: 1711–1716 (2007)

    Article  CAS  Google Scholar 

  10. Kim KN, Heo SJ, Kang SM, Ahn G, Jeon YJ. Fucoxanthin induces apoptosis in human leukemia HL-60 cells through a ROS-mediated Bcl-xL pathway. Toxicol. In Vitro 24: 1648–1654 (2010)

    Article  CAS  Google Scholar 

  11. Das SK, Ren R, Hashimoto T, Kanazawa K. Fucoxanthin induces apoptosis in osteoclast-like cells differentiated from RAW 264.7 cells. J. Agr. Food Chem. 58: 6090–6095 (2010)

    Article  CAS  Google Scholar 

  12. Heo SJ, Yoon WJ, Kim KN, Ahn GN, Kang SM, Kang DH, Affan A, Oh C, Jung Wk, Jeon YJ. Evaluation of anti-inflammatory effect of fucoxanthin isolated from brown algae in lipopolysaccharide-stimulated RAW 264.7 macrophages. Food Chem. Toxicol. 48: 2045–2051 (2010)

    Article  CAS  Google Scholar 

  13. Kim IH, Lee JH. Antimicrobial activities against methicillin-resistant Staphylococcus aureus from macroalgae. J. Ind. Eng. Chem. 14: 568–572 (2008)

    Article  CAS  Google Scholar 

  14. Heo SJ, Park EJ, Lee KW, Jeon YJ. Antioxidant activities of enzymatic extracts from brown seaweeds. Bioresource Technol. 96: 1613–1623 (2005)

    Article  CAS  Google Scholar 

  15. Iwashima M, Mori J, Ting X, Matsunaga T, Hayashi K, Shinoda D, Saito H, Sankawa U, Hayashi T. Antioxidant and antiviral activities of plastoquinones from the brown alga Sargassum micracanthum, and a new chromene derivative converted from the plastoquinones. Biol. Pharm. Bull. 28: 374–377 (2005)

    Article  CAS  Google Scholar 

  16. Lee SY, Song EJ, Kim KBWR, Lim SI, Hong YK, Ahn DH. Antimicrobial activity of Myagropsis myagroides and interaction with food composition. Food Sci. Biotechnol. 20: 85–92 (2011)

    Article  Google Scholar 

  17. Wong CK, Ooi VEC, Ang PO. Protective effects of seaweeds against liver injury caused by carbon tetrachloride in rats. Chemosphere 41: 173–176 (2000)

    Article  CAS  Google Scholar 

  18. Lee JH, Yang HS, Park KW, Kim JY, Lee MK, Jeong IY, Shim KH, Kim YS, Yamada K, Seo KI. Mechanisms of thiosulfinates from Allium tuberosum L.-induced apoptosis in HT-29 human colon cancer cells. Toxicol. Lett. 188: 142–147 (2009)

    Article  CAS  Google Scholar 

  19. Kim SY, Park KW, Kim JY, Jeong IY, Byun MW, Park JE, Yee ST, Kim KH, Rhim JS, Yamada K, Seo KI. Thiosulfinates from Allium tuberosum L. induce apoptosis via caspase-dependent and — independent pathways in PC-3 human prostate cancer cells. Bioorg. Med. Chem. Lett. 18: 199–204 (2008)

    Article  CAS  Google Scholar 

  20. Park SY, Cho SJ, Kwon HC, Lee KR, Rhee DK, Pyo S. Caspaseindependent cell death by allicin in human epithelial carcinoma cells: Involvement of PKA. Cancer Lett. 224: 123–132 (2005)

    Article  CAS  Google Scholar 

  21. Yoshikawa N, Yamada J, Tsuno NH, Okaji Y, Kawai K, Tsuchiya T, Yoneyama S, Tanaka J, Shuno Y, Nishikawa T, Nagawa H, Oshima N, Takahashi K. Plaunotol and geranylgeraniol induce caspasemediated apoptosis in colon cancer. J. Surg. Res. 153: 246–253 (2009)

    Article  CAS  Google Scholar 

  22. Kim JE, Chung WY, Chun KS, Lee CK, Park HJ, Kim WB, Park KK. Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells. Biosci. Biotech. Bioch. 74: 788–792 (2010)

    Article  CAS  Google Scholar 

  23. Lee H, Kang C, Jung ES, Kim JS, Kim E. Antimetastatic activity of polyphenol-rich extract of Ecklonia cava through the inhibition of the Akt pathway in A549 human lung cancer cells. Food Chem. 127: 1229–1236 (2011)

    Article  CAS  Google Scholar 

  24. Zhang Z, Zhang P, Hamada M, Takahashi S, Xing G, Liu J, Sugiura N. Potential chemoprevention effect of dietary fucoxanthin on urinary bladder cancer EJ-1 cell line. Oncol. Rep. 20: 1099–1103 (2008)

    CAS  Google Scholar 

  25. Yamamoto K, Ishikawa C, Katano H, Yasumoto T, Mori N. Fucoxanthin and its deacetylated product, fucoxanthinol, induce apoptosis of primary effusion lymphomas. Cancer Lett. 300: 225–234 (2011)

    Article  CAS  Google Scholar 

  26. Kim KY, Yu SN, Lee SY, Chun SS, Choi YL, Park YM, Song CS, Chatterjee B, Ahn SC. Salinomycin-induced apoptosis of human prostate cancer cells due to accumulated reactive oxygen species and mitochondrial membrane depolarization. Biochem. Bioph. Res. Co. 413: 80–86 (2011)

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Il Yun Jeong.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cho, B.O., Ryu, H.W., So, Y. et al. Induction of apoptosis by Myagropsis myagroides extracts is associated with a caspase dependent pathway and reactive oxygen species generation in human cancer cells. Food Sci Biotechnol 21, 1367–1375 (2012). https://doi.org/10.1007/s10068-012-0180-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10068-012-0180-4

Keywords

Navigation