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Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation

  • Published:
Molecules and Cells

Abstract

Fuel ethanol production is far more costly to produce than fossil fuels. There are a number of approaches to costeffective fuel ethanol production from biomass. We characterized stress response of thermotolerant Saccharomyces cerevisiae KNU5377 during glucose-based batch fermentation at high temperature (40°C). S. cerevisiae KNU5377 (KNU5377) transcription factors (Hsf1, Msn2/4, and Yap1), metabolic enzymes (hexokinase, glyceraldehyde-3-phosphate dehydrogenase, glucose-6-phosphate dehydrogenase, isocitrate dehydrogenase, and alcohol dehydrogenase), antioxidant enzymes (thioredoxin 3, thioredoxin reductase, and porin), and molecular chaperones and its cofactors (Hsp104, Hsp82, Hsp60, Hsp42, Hsp30, Hsp26, Cpr1, Sti1, and Zpr1) are upregulated during fermentation, in comparison to S. cerevisiae S288C (S288C). Expression of glyceraldehyde-3-phosphate dehydrogenase increased significantly in KNU5377 cells. In addition, cellular hydroperoxide and protein oxidation, particularly lipid peroxidation of triosephosphate isomerase, was lower in KNU5377 than in S288C. Thus, KNU5377 activates various cell rescue proteins through transcription activators, improving tolerance and increasing alcohol yield by rapidly responding to fermentation stress through redox homeostasis and proteostasis.

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Authors and Affiliations

  1. Advanced Bio-resource Research Center, Kyungpook National University, Daegu, 702-701, Korea

    Il-Sup Kim & Ho-Sung Yoon

  2. Department of Biology, Kyungpook National University, Daegu, 702-701, Korea

    Young-Saeng Kim & Ho-Sung Yoon

  3. Department of Microbiology, Kyungpook National University, Daegu, 702-701, Korea

    Hyun Kim & Ingnyol Jin

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  1. Il-Sup Kim
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  2. Young-Saeng Kim
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  3. Hyun Kim
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  4. Ingnyol Jin
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  5. Ho-Sung Yoon
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Correspondence to Ho-Sung Yoon.

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Kim, IS., Kim, YS., Kim, H. et al. Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation. Mol Cells 35, 210–218 (2013). https://doi.org/10.1007/s10059-013-2258-0

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  • DOI: https://doi.org/10.1007/s10059-013-2258-0

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