Abstract
Graphene has attracted substantial attention due to its advantageous materialistic applicability. In the present study, we tested the biocompatibility of graphene films synthesized by chemical vapor deposition with electrogenic primary adult cardiac cells (cardiomyocytes) by measuring the cell properties such as cell attachment, survival, contractility and calcium transients. The results show that the graphene films showed stable cell attachment and excellent biocompatibility with the electrogenic cardiomyocytes, suggesting their useful applications for future cell biology studies.
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Beck, K., Hunter, I., and Engel, J. (1990). Structure and function of laminin: anatomy of a multidomain glycoprotein. FASEB J. 4, 148–160.
Bird, S.D., Doevendans, P.A., van Rooijen, M.A., Brutel de la Riviere, A., Hassink, R.J., Passier, R., and Mummery, C.L. (2003). The human adult cardiomyocyte phenotype. Cardiovasc. Res. 58, 423–434.
Bitounis, D., Ali-Boucetta, H., Hong, B.H., Min, D.H., and Kostarelos, K. (2013). Prospects and challenges of graphene in biomedical applications. Adv. Mater. 25, 2258–2268.
Cohen-Karni, T., Qing, Q., Li, Q., Fang, Y., and Lieber, C.M. (2010) Graphene and nanowire transistors for cellular interfaces and electrical recording. Nano Lett. 10, 1098–1102.
Duch, M.C., Budinger, G.R., Liang, Y.T., Soberanes, S., Urich, D., Chiarella, S.E., Campochiaro, L.A., Gonzalez, A., Chandel, N.S., Hersam, M.C., et al. (2011). Minimizing oxidation and stable nanoscale dispersion improves the biocompatibility of graphene in the lung. Nano Lett. 11, 5201–5207.
Freire, E., Gomes, F.C., Linden, R., Neto, V.M., and Coelho-Sampaio, T. (2002). Structure of laminin substrate modulates cellular signaling for neuritogenesis. J. Cell Sci. 115, 4867–4876.
Geim, A.K. (2009). Graphene: status and prospects. Science 324, 1530–1534.
Geim, A.K., and Novoselov, K.S. (2007). The rise of graphene. Nat. Mater. 6, 183–191.
Gregory, K.N., Ginsburg, K.S., Bodi, I., Hahn, H., Marreez, Y.M., Song, Q., Padmanabhan, P.A., Mitton, B.A., Waggoner, J.R., Del Monte, F., et al. (2006). Histidine-rich Ca binding protein: a regulator of sarcoplasmic reticulum calcium sequestration and cardiac function. J. Mol. Cell Cardiol. 40, 653–665.
Hess, L.H., Jansen, M., Maybeck, V., Hauf, M.V., Seifert, M., Stutzmann, M., Sharp, I.D., Offenhausser, A., and Garrido, J.A. (2011). Graphene transistor arrays for recording action potentials from electrogenic cells. Adv. Mater. 23, 5045–5049, 4968.
Jang, H., Ryoo, S.R., Lee, M.J., Han, S.W., and Min, D.H. (2013). A new helicase assay based on graphene oxide for anti-viral drug development. Mol. Cells 35, 269–273.
Jo, G., Choe, M., Cho, C.Y., Kim, J.H., Park, W., Lee, S., Hong, W.K., Kim, T.W., Park, S.J., Hong, B.H., et al. (2010a). Largescale patterned multi-layer graphene films as transparent conducting electrodes for GaN light-emitting diodes. Nanotechnology 21, 175201.
Jo, G., Na, S.-I., Oh, S.-H., Lee, S., Kim, T.-S., Wang, G., Choe, M., Park, W., Yoon, J., Kim, D.-Y., et al. (2010b). Tuning of a graphene-electrode work function to enhance the efficiency of organic bulk heterojunction photovoltaic cells with an inverted structure. Appl. Phys. Lett. 97, 213301.
Kahng, Y.H., Lee, S., Choe, M., Jo, G., Park, W., Yoon, J., Hong, W.K., Cho, C.H., Lee, B.H., and Lee, T. (2011). A study of graphene films synthesized on nickel substrates: existence and origin of small-base-area peaks. Nanotechnology 22, 045706.
Kalbacova, M., Broz, A., Kong, J., and Kalbac, M. (2010). Graphene substrates promote adherence of human osteoblasts and mesenchymal stromal cells. Carbon 48, 4323–4329.
Kwon, S.J., and Kim, D.H. (2009). Characterization of junctate-SERCA2a interaction in murine cardiomyocyte. Biochem. Biophys. Res. Commun. 390, 1389–1394.
Lee, S., Jo, G., Kang, S.J., Wang, G., Choe, M., Park, W., Kim, D.Y., Kahng, Y.H., and Lee, T. (2011). Enhanced charge injection in pentacene field-effect transistors with graphene electrodes. Adv. Mater. 23, 100–105.
Li, N., Zhang, X., Song, Q., Su, R., Zhang, Q., Kong, T., Liu, L., Jin, G., Tang, M., and Cheng, G. (2011). The promotion of neurite sprouting and outgrowth of mouse hippocampal cells in culture by graphene substrates. Biomaterials 32, 9374–9382.
Mao, X., Su, H., Tian, D., Li, H., and Yang, R. (2013). Bipyrenefunctionalized graphene as a “turn-on” fluorescence sensor for manganese(II) ions in living cells. ACS Appl. Mater. Interfaces 5, 592–597.
Oh, J.G., Jeong, D., Cha, H., Kim, J.M., Lifirsu, E., Kim, J., Yang, D.K., Park, C.S., Kho, C., Park, S., et al. (2012). PICOT increases cardiac contractility by inhibiting PKCzeta activity. J. Mol. Cell Cardiol. 53, 53–63.
Park, C.S., Chen, S., Lee, H., Cha, H., Oh, J.G., Hong, S., Han, P., Ginsburg, K.S., Jin, S., Park, I., et al. (2013). Targeted ablation of the histidine-rich Ca(2+)-binding protein (HRC) gene is associated with abnormal SR Ca(2+)-cycling and severe pathology under pressure-overload stress. Basic Res. Cardiol. 108, 344.
Ryoo, S.R., Kim, Y.K., Kim, M.H., and Min, D.H. (2010). Behaviors of NIH-3T3 fibroblasts on graphene/carbon nanotubes: proliferation, focal adhesion, and gene transfection studies. ACS Nano 4, 6587–6598.
Sahni, D., Jea, A., Mata, J.A., Marcano, D.C., Sivaganesan, A., Berlin, J.M., Tatsui, C.E., Sun, Z., Luerssen, T.G., Meng, S., et al. (2013). Biocompatibility of pristine graphene for neuronal interface. J. Neurosurg. Pediatr. 11, 575–583.
Yamamoto, T., Yano, M., Kohno, M., Hisaoka, T., Ono, K., Tanigawa, T., Saiki, Y., Hisamatsu, Y., Ohkusa, T., and Matsuzaki, M. (1999). Abnormal Ca2+ release from cardiac sarcoplasmic reticulum in tachycardia-induced heart failure. Cardiovasc. Res. 44, 146–155.
Yarnitzky, T., and Volk, T. (1995). Laminin is required for heart, somatic muscles, and gut development in the Drosophila embryo. Dev. Biol. 169, 609–618.
Yoon, J., Choi, S.C., Park, C.Y., Choi, J.H., Kim, Y.I., Shim, W.J., and Lim, D.S. (2008). Bone marrow-derived side population cells are capable of functional cardiomyogenic differentiation. Mol. Cells 25, 216–223.
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Kim, T., Kahng, Y.H., Lee, T. et al. Graphene films show stable cell attachment and biocompatibility with electrogenic primary cardiac cells. Mol Cells 36, 577–582 (2013). https://doi.org/10.1007/s10059-013-0277-5
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DOI: https://doi.org/10.1007/s10059-013-0277-5