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Repair of segmental radial defects in dogs using tailor-made titanium mesh cages with plates combined with calcium phosphate granules and basic fibroblast growth factor-binding ion complex gel

  • Original Article
  • Artificial Skin, Muscle, Bone / Joint, Neuron
  • Published:
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Abstract

Repair of large segmental defects of long bones are a tremendous challenge that calls for a novel approach to supporting immediate weight bearing and bone regeneration. This study investigated the functional and biological characteristics of a combination of a tailor-made titanium mesh cage with a plate (tTMCP) with tetrapod-shaped alpha tricalcium phosphate granules (TB) and basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) to repair 20-mm segmental radial defects in dogs. The defects were created surgically in 18 adult beagle dogs and treated by implantation of tTMCPs with TB with (TB-gel group) or without (TB group) f-IC gel. Each tTMCP fitted the defect well, and all dogs could bear weight on the affected limb immediately after surgery. Dogs were euthanized 4, 8 and 24 weeks after implantation. Histomorphometry showed greater infiltration of new vessels and higher bone union rate in the TB-gel group than in the TB group. The lamellar bone volume and mineral apposition rate did not differ significantly between the groups, indicating that neovascularization may be the primary effect of f-IC gel on bone regeneration. This combination method which is tTMCP combined with TB and f-IC gel, would be useful for the treatment of segmental long bone defects.

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Acknowledgments

This research was supported by the Japan Society for the Promotion of Science (JSPS) through the “Founding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program),” which was initiated by the Council for Science and Technology Policy (CSTP), and also by Research and Development Programs for Three-dimensional Complex Organ Structures and for Autonomous Regeneration Devices from the New Energy and Industrial Technology Development Organization (NEDO). We sincerely thank Dr. Akemi Ito, Ito Bone Histomorphometry Institute, Niigata, Japan for her invaluable assistance.

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Authors and Affiliations

  1. Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Muneki Honnami, I-li Liu, Ryohei Nishimura, Nobuo Sasaki & Manabu Mochizuki

  2. Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Muneki Honnami & Ung-il Chung

  3. Division of Clinical Biotechnology, Graduate School of Medicine, Center for Disease Biology and Integrative Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Sungjin Choi, Hironori Hojo, Nobuyuki Shimohata, Shinsuke Ohba & Ung-il Chung

  4. Division of Tissue Engineering, Department of Vascular Regeneration, The University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan

    Wataru Kamimura & Hiroyuki Koyama

  5. Nanoscale Materials Division, Biomaterials Unit, Smart Biomaterials Group, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan

    Wataru Kamimura & Tetsushi Taguchi

  6. Matsuura Machinery Corporation, 1-1 Urushihara-cho, Fukui, 910-8530, Japan

    Makoto Ichimura, Yukinori Urushisaki & Koichi Amaya

Authors
  1. Muneki Honnami
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  2. Sungjin Choi
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  3. I-li Liu
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  4. Wataru Kamimura
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  5. Tetsushi Taguchi
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  6. Makoto Ichimura
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  7. Yukinori Urushisaki
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  8. Hironori Hojo
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  9. Nobuyuki Shimohata
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  10. Shinsuke Ohba
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  11. Koichi Amaya
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  12. Hiroyuki Koyama
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  13. Ryohei Nishimura
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  14. Ung-il Chung
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  15. Nobuo Sasaki
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  16. Manabu Mochizuki
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Corresponding author

Correspondence to Manabu Mochizuki.

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Honnami, M., Choi, S., Liu, Il. et al. Repair of segmental radial defects in dogs using tailor-made titanium mesh cages with plates combined with calcium phosphate granules and basic fibroblast growth factor-binding ion complex gel. J Artif Organs 20, 91–98 (2017). https://doi.org/10.1007/s10047-016-0918-5

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  • DOI: https://doi.org/10.1007/s10047-016-0918-5

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