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A novel cell-containing device for regenerative medicine: biodegradable nonwoven filters with peripheral blood cells promote wound healing

  • Original Article
  • Tissue Engineering / Regenerative Medicine
  • Published:
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Abstract

The efficacy of skin regeneration devices consisting of nonwoven filters and peripheral blood cells was investigated for wound healing. We previously found that human peripheral blood cells enhanced their production of growth factors, such as transforming growth factor β1 (TGF-β1) and vascular endothelial growth factor, when they were captured on nonwoven filters. Cells on biodegradable filters were expected to serve as a local supply of growth factors and cell sources when they were placed in wounded skin. Nonwoven filters made of biodegradable polylactic acid (PLA) were cut out as 13-mm disks and placed into cell-capturing devices. Mouse peripheral blood was filtered, resulting in PLA filters with mouse peripheral blood cells (m-PBCs) at capture rates of 65.8 ± 5.2 %. Then, the filters were attached to full-thickness surgical wounds in a diabetic db/db mouse skin for 14 days as a model of severe chronic wounds. The wound area treated with PLA nonwoven filters with m-PBCs (PLA/B+) was reduced to 8.5 ± 12.2 % when compared with day 0, although the non-treated control wounds showed reduction only to 60.6 ± 27.8 %. However, the PLA filters without m-PBCs increased the wound area to 162.9 ± 118.7 %. By histopathological study, the PLA/B+ groups more effectively accelerated formation of epithelium. The m-PBCs captured on the PLA filters enhanced keratinocyte growth factor (FGF-7) and TGF-β1 productions in vitro, which may be related to wound healing. This device is useful for regeneration of wounded skin and may be adaptable for another application.

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Acknowledgments

The authors thank Ms. Mikiko Ariga of Asahi Kasei Fibers Corporation for providing the nonwoven filters. The authors thank Ms. Miwa Sakata, and Mr. Masaya Yamamoto, Kazuki Kawamura and Tomoya Oguri for their technical assistance. This work was partly supported by a research grant from Asahi Kasei Corporation and JSPS KAKENHI Grant Number 25410232.

Conflict of interest

Mikitomo Yasutake, General Manager, and Ushio Iwamoto, Chief Researcher, are employees of Asahi Kasei Corporation Co., Ltd. (Cell Therapy Technology, Healthcare R&D Center) and have the stock of the company. Yasuo Tokushima is an employee of Asahi Kasei Medical Co., Ltd. (Manager of Scientific and Technical Department, Japan Operation Division, Blood Purification Business Unit), and has the stock of Asahi Kasei Corporation Co., Ltd. Nobuya Kitaguchi has the stock of Asahi Kasei Corporation Co., Ltd. Nobuya Kitaguchi and Hideo Hori received the research funding from Asahi Kasei Corporation Co., Ltd. The other authors declare that they have no conflict of interest.

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Author notes

  1. Yoshihiro Takami

    Present address: Japan Labour Health and Welfare Organization, Tokyo Rosai Hospital, Tokyo, Japan

Authors and Affiliations

  1. Healthcare R&D Center, Asahi Kasei Corporation, Tokyo, Japan

    Ushio Iwamoto & Mikitomo Yasutake

  2. Faculty of Clinical Engineering, School of Health Sciences, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan

    Hideo Hori & Nobuya Kitaguchi

  3. Department of Plastic Surgery, School of Medicine, Kyorin University, Tokyo, Japan

    Yoshihiro Takami

  4. Therapeutic Apheresis Division, Asahi Kasei Medical, Co., Ltd, Tokyo, Japan

    Yasuo Tokushima

  5. Faculty of Medical Management and Information Science, School of Health Sciences, Fujita Health University, Toyoake, Japan

    Masanori Shinzato

Authors
  1. Ushio Iwamoto
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  2. Hideo Hori
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  3. Yoshihiro Takami
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  4. Yasuo Tokushima
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  5. Masanori Shinzato
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  6. Mikitomo Yasutake
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  7. Nobuya Kitaguchi
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Corresponding authors

Correspondence to Hideo Hori or Nobuya Kitaguchi.

Additional information

U. Iwamoto and H. Hori contributed equally.

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Iwamoto, U., Hori, H., Takami, Y. et al. A novel cell-containing device for regenerative medicine: biodegradable nonwoven filters with peripheral blood cells promote wound healing. J Artif Organs 18, 315–321 (2015). https://doi.org/10.1007/s10047-015-0845-x

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  • DOI: https://doi.org/10.1007/s10047-015-0845-x

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