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The “kidney–liver” multiorgan ex vivo perfused model improves the circuit’s biochemical milieu during perfusion compared to the “liver–kidney” counterpart

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  • Artificial Liver, Pancreas
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Abstract

The multiorgan ex vivo perfused liver–kidney model allows studying the hepatic pathophysiology and purifying waste products. We tested if the addition of the kidney first followed by the liver (KL circuit) produces better results compared to the classic liver-first approach (LK). Intact livers and kidneys were obtained post mortem from ten female domestic white pigs, five experiments were conducted with the KL circuit and five with the LK. Bile, urine production, arterial blood gases, glucose, renal and liver tests were collected hourly during the perfusions. The KL circuit had values more close to physiological ranges, more stable over time and showed less variability compared to the LK circuit for urine production, glucose, PH, anion gap, lactate, urea, sodium, potassium and Alanine Transaminase (ANOVA test for repeated measures p < 0.05). The KL circuit produced a more physiological and reliable biochemical milieu.

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Acknowledgments

We would like to thank Sarah Hosgood from the Department of Transplantation, University Hospitals of Leicester, for connecting the porcine kidney in this model and providing expert advice during the experiments setup.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Hepatobiliary and Pancreatic Surgery, Leicester General Hospital, University of Leicester, Gwendolen Road, Leicester, LE5 4PW, UK

    Wen Yuan Chung, Gianpiero Gravante, Amar Eltweri, Seok Ling Ong, Cristina Pollard, Mathew Metcalfe & Ashley Dennison

  2. Laboratory of Biometry, Department of Human Physiology, University of Tor Vergata, Rome, Italy

    Roberto Sorge

Authors
  1. Wen Yuan Chung
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  2. Gianpiero Gravante
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  3. Amar Eltweri
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  4. Roberto Sorge
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  5. Seok Ling Ong
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  6. Cristina Pollard
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  7. Mathew Metcalfe
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  8. Ashley Dennison
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Correspondence to Gianpiero Gravante.

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Chung, W.Y., Gravante, G., Eltweri, A. et al. The “kidney–liver” multiorgan ex vivo perfused model improves the circuit’s biochemical milieu during perfusion compared to the “liver–kidney” counterpart. J Artif Organs 18, 151–161 (2015). https://doi.org/10.1007/s10047-014-0813-x

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  • DOI: https://doi.org/10.1007/s10047-014-0813-x

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