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Phase and fluorescence imaging by combination of digital holographic microscopy and fluorescence microscopy

  • Special Section: Regular Paper
  • Biomedical Imaging and Sensing Conference (BISC'14), Yokohama, Japan
  • Published:
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Abstract

Hybrid digital holographic microscopy that combines fluorescence microscopy and digital holographic microscopy into a single system for biological applications is proposed. In the proposed system, a phase image and a fluorescence image can be obtained simultaneously by selecting the different wavelengths of the fluorescent light and the phase measurement. Especially for biological applications, the cell structure can be obtained by the phase imaging based on digital holography and nucleus of the cell can be obtained by the fluorescence imaging. The measurement of fluorescence beads and egera densa presented the feasibility of simultaneous detection of both a phase image and a fluorescence image.

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References

  1. J.W. Goodman, R.W. Lawrence, Digital image formation from electronically detected holograms. Appl. Phys. Lett. 11, 77–79 (1967)

    Article  ADS  Google Scholar 

  2. U. Schnars, W. Juptner, Direct recording of holograms by a CCD target and numerical reconstruction. Appl. Opt. 33, 179–181 (1994)

    Article  ADS  Google Scholar 

  3. B. Kemper, G. von Bally, Digital holographic microscopy for live cell applications and technical inspection. Appl. Opt. 47, A52–A61 (2008)

    Article  ADS  Google Scholar 

  4. M.K. Kim, Principles and techniques of digital holographic microscopy. SPIE Rev. 1, 018005 (2010)

    Google Scholar 

  5. Y. Frauel, T.J. Naughton, O. Matoba, E. Tajahuerce, B. Javidi, Three-dimensional imaging and processing using computational holographic imaging. Proc. IEEE 94, 636–653 (2006)

    Article  Google Scholar 

  6. T. Tahara, K. Ito, T. Kakue, M. Fujii, Y. Shimozato, Y. Awatsuji, K. Nishio, S. Ura, T. Kubota, O. Matoba, Parallel phase-shifting digital holographic microscopy. Biomed. Opt. Express 1, 610–616 (2010)

    Article  Google Scholar 

  7. T. Tahara, R. Yonesaka, S. Yamamoto, T. Kakue, P. Xia, Y. Awatsuji, K. Nishio, S. Ura, T. Kubota, O. Matoba, High-speed three-dimensional microscope for dynamically moving biological objects based on parallel phase-shifting digital holographic microscopy. IEEE J. Sel. Topics Quantum Electron. 18, 1387–1393 (2012)

    Article  Google Scholar 

  8. W. Osten, A. Faridian, P. Gao, L. Korner, D. Naik, G. Pedrini, A.K. Singh, M. Takeda, M. Wilke, Recent advances in digital holography. Appl. Opt. 53, G44–G63 (2014)

    Article  Google Scholar 

  9. J. Rosen, G. Brooker, Non-scanning motionless fluorescence three-dimensional holographic micoroscopy. Nat. Photon. 2, 190–195 (2008)

    Article  ADS  Google Scholar 

  10. G. Brooker, N. Siegel, V. Wang, J. Rosen, Optimal resolution of Fresnel incoherent correlation holographic fluorescence microscopy. Opt. Express 19, 5047–5062 (2011)

    Article  ADS  Google Scholar 

  11. Y.K. Park, G. Popescu, K. Badizadegan, R.R. Dasari, M.S. Feld, Diffraction phase and fluorescence microscopy. Opt. Express 14, 8263–8268 (2006)

    Article  ADS  Google Scholar 

  12. E. Shaffer, N. Pavillon, C. Depeursinge, Single-shot, simultaneous incoherent and holographic microscopy. J. Microsc. 245(1), 49–62 (2012)

    Article  Google Scholar 

  13. M. Takeda, H. Ina, S. Kobayashi, Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry. J. Opt. Soc. Am. 72, 156–160 (1982)

    Article  ADS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Systems Science, Graduate School of System Informatics, Kobe University, Rokkodai 1-1, Nada, Kobe, 657-8501, Japan

    Xiangyu Quan, Kouichi Nitta & Osamu Matoba

  2. Department of Designing Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan

    Peng Xia

  3. Division of Electronics, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan

    Yasuhiro Awatsuji

Authors
  1. Xiangyu Quan
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  2. Kouichi Nitta
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  3. Osamu Matoba
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  4. Peng Xia
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  5. Yasuhiro Awatsuji
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Correspondence to Xiangyu Quan.

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Quan, X., Nitta, K., Matoba, O. et al. Phase and fluorescence imaging by combination of digital holographic microscopy and fluorescence microscopy. Opt Rev 22, 349–353 (2015). https://doi.org/10.1007/s10043-015-0042-4

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  • DOI: https://doi.org/10.1007/s10043-015-0042-4

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