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Impedimetric evaluation of hybrid cationic porphyrin/quantum dot multilayer assemblies: a biocompatible interface for calf thymus DNA immobilization

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Abstract

In this work, a cationic porphyrin, ascribed as [Ttolyl(P-(C6H5)3)4]4+, where Ttolyl = 5,10,15,20 tetrakistolylporphyrin, was electrostatically assembled with CdTe quantum dots capped with glutathione (GSH) of diameter ~3.28 nm via a layer-by-layer methodology. This multilayer assembly was evaluated as a biocompatible interface with synergic effects for calf thymus double-stranded DNA (CT DNA) immobilization, considering that the hybrid assembly contains a cationic porphyrin with a high binding constant for CT DNA, and quantum dots with polypeptide as a capping agent. The multilayer assembly, ascribed as ITO/{[Ttolyl(P-(C6H5)3)4]4+/CdTe} n (n = 1–5), was characterized by UV-Vis spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS). The last technique was explored in order to check the adsorption of CT DNA onto the multilayer porphyrin/quantum dot assembly. The difference in electron transfer resistance (ΔR ct) obtained after CT DNA incubations showed the best result for a specific multilayer assembly, n = 3. {[Ttolyl(P-(C6H5)3)4]4+/CdTe}3 showed a good correlation of ΔR ct with the logarithmic concentration of CT DNA, in the range 1.0 × 10−10 to 1.0 × 10−6 M with a limit of detection (LOD) of 1.5 × 10−12 M. Conversely, when the {[Ttolyl(P-(C6H5)3)4]4+/CdTe}3 system was incubated with calf thymus single-stranded DNA and salmon testes DNA, no significant difference in ΔR ct was observed. We conclude that the newly described {[Ttolyl(P-(C6H5)3)4]4+/CdTe} n system is a choice method for the impedimetric determination of CT DNA.

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Acknowledgments

Camilo García thanks the FONDECYT program regarding Initiation FONDECYT grant 11150434 and Postdoctoral Project 3130328. M. J Aguirre expresses thanks regarding FONDECYT grant 1160324.

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Authors and Affiliations

  1. Facultad de Química y Biología, Departamento de Química de los Materiales, Laboratorio de Polímeros Conductores, Universidad de Santiago de Chile, Avenida Libertador Bernardo O’Higgins, 3363, Estación Central, Chile

    Camilo García, Freddy Navarro & María Jesús Aguirre

  2. Facultad de Química y Biología, Departamento de Química de los Materiales, Laboratorio de Electroquímica del Estado Sólido, Universidad de Santiago de Chile, Avenida Libertador Bernardo O’Higgins, 3363, Estación Central, Chile

    Domingo Ruíz-León

  3. Facultad de Ciencias Naturales y Matemáticas, Universidad de Ibagué, Carrera 22 Calle 67, Ibagué, 730001, Colombia

    Olimpo García Beltrán

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  1. Camilo García
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Correspondence to Camilo García.

Electronic supplementary material

Figure S1

Cyclic voltammetry 2 mM [Ru(NH3)5Cl]Cl2 in 10 mM Tris, pH = 7.0. Scan rate 50 mv/s. (GIF 64 kb)

High resolution image (GIF 64 kb) (TIFF 7611 kb)

Fig. S2

Nyquist plot of ITO/{[Ttolyl(P-(C6H5)3)4]4+/CdTe}n, n = 1–5, in the presence of 2.0 mM [Ru(NH3)5Cl]Cl2, 10.0 mM Tris with a pH of 7.0, and 0.1 M KCl. (GIF 60 kb)

High resolution image (TIFF 7611 kb)

Figure S3

UV-Vis spectra of ITO/{[Ttolyl(P-(C6H5)3)4]4+/CdTe (d = 3.28 nm)}3 after incubation with different genomic DNA at the same concentration of 1.0 × 10−6 M. (GIF 60 kb)

High resolution image (TIFF 7611 kb)

Figure S4

Cyclic voltammetry of ITO/{[Ttolyl(P-(C6H5)3)4]4+/CdTe (d = 3.28 nm)}3 after incubation with different genomic DNA in 10 mM Tris, pH = 7.0. Scan rate 50 mv/s. (GIF 50 kb)

High resolution image (TIFF 7611 kb)

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García, C., Navarro, F., Ruíz-León, D. et al. Impedimetric evaluation of hybrid cationic porphyrin/quantum dot multilayer assemblies: a biocompatible interface for calf thymus DNA immobilization. J Solid State Electrochem 21, 243–253 (2017). https://doi.org/10.1007/s10008-016-3367-4

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  • DOI: https://doi.org/10.1007/s10008-016-3367-4

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