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Direct electrochemical analysis of glucose oxidase on a graphene aerogel/gold nanoparticle hybrid for glucose biosensing

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Abstract

Homogeneously distributed self-assembling graphene aerogel/gold nanoparticle (GA/GNs) hybrid materials with three-dimensional (3D) interconnected pores were fabricated through a simple hydrothermal route. A highly sensitive glucose oxidase (GOD) sensor is then developed based on the GA/GN hybrid materials. The obtained porous structure of the GA/GNs hybrid favored high-density immobilization of the enzyme and penetration of water-soluble molecules, which provided a biocompatible sensing platform for GOD immobilization. This GOD biosensor exhibits remarkable sensitivity (257.60 μA · mM−1 · cm−2), an approximate linear detection range of glucose concentration (50 μmol · L−1 to 450 μmol · L−1), and a detection limit of 0.597 μmol · L−1. These results indicate that the in situ encapsulation of different nanomaterials into a 3D graphene aerogel framework can enable the fabrication of a series of graphene-based 3D porous materials with promising applications.

We have demonstrated a novel glucose sensor based on porous structure of the GA/GNs hybrid, which favored high-density immobilization of the enzyme, penetration of water-soluble molecules, and providing a biocompatible sensing platform for GOD immobilization.

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Acknowledgments

This work was financially supported by the National Basic Research Program of China (973 Program: 2013CB932903), the National Science Foundations of China (No. 61205057), China Postdoctoral Science special Foundation (2012 T50488), China Postdoctoral Science Foundation (2011 M500896), Jiangsu Planned Projects for Postdoctoral Research Funds (1102015C), Natural Science Foundation of Education Bureau of Jiangsu Province (12KJB180009), the Scientific Research Foundation of Nanjing University of Posts and Telecommunications (NY213184), and the open research fund of Key Laboratory of MEMS of Ministry of Education, Southeast University.

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Authors and Affiliations

  1. School of Geography and Biological Information, Nanjing University of Posts and Telecommunications, Nanjing, 210023, People’s Republic of China

    Bojun Wang & Shancheng Yan

  2. National Laboratory of Solid State Microstructures School of Electronic Science and Engineering, Nanjing University, Nanjing, 210093, People’s Republic of China

    Shancheng Yan & Yi Shi

Authors
  1. Bojun Wang
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  2. Shancheng Yan
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  3. Yi Shi
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Correspondence to Shancheng Yan.

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Wang, B., Yan, S. & Shi, Y. Direct electrochemical analysis of glucose oxidase on a graphene aerogel/gold nanoparticle hybrid for glucose biosensing. J Solid State Electrochem 19, 307–314 (2015). https://doi.org/10.1007/s10008-014-2608-7

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  • DOI: https://doi.org/10.1007/s10008-014-2608-7

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