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Chondroitin sulfate prevents peritoneal fibrosis in mice by suppressing NF-κB activation

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Abstract

Long-term peritoneal dialysis causes peritoneal fibrosis, and previous reports suggest that inflammation plays a critical role in peritoneal fibrosis. Chondroitin sulfate (CS) suppresses the inflammatory response by preventing activation of nuclear factor (NF)-κB. We examined the effect of CS on the peritoneal fibrosis induced by chlorhexidine gluconate (CG) in mice. CS or water was administered daily. We divided mice into four groups: administered vehicle and water (control); administered vehicle and CS (CS); administered CG and water (CG); and administered CG and CS (CG+CS). Morphologic changes were assessed by Masson’s trichrome staining. Inflammation- and fibrosis-associated factors were assessed by immunohistochemistry. Activation of NF-κB was examined by southwestern histochemistry. CS administration suppressed the progression of submesothelial thickening. The numbers of inflammation- and fibrosis-associated factors -positive cells were significantly decreased in the CG+CS group, compared to the CG group. Based on SWH, the CG+CS group contained significantly fewer NF-κB-activated cells than the CG group. Our results indicate that CS suppresses peritoneal fibrosis via suppression of NF-κB activation. These results suggest that CS has therapeutic potential for peritoneal fibrosis.

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Acknowledgments

This work was supported by Grants from the Japanese Association of Dialysis Physicians (No. 25067). We would like to thank Ms. Ryoko Yamamoto and Ms. Ayako Matsuo for the excellent experimental assistance.

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Authors and Affiliations

  1. Department of Nephrology, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki, Japan

    Shinichi Abe, Yoko Obata, Satoru Oka & Tomoya Nishino

  2. Department of infectious disease, Unit of molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

    Shinichi Abe & Koichi Izumikawa

  3. Medical Education Development Center, Nagasaki University Hospital, Nagasaki, Japan

    Yoko Obata

  4. Unit of Basic Medical Science, Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

    Takehiko Koji

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  1. Shinichi Abe
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  2. Yoko Obata
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  5. Tomoya Nishino
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Correspondence to Yoko Obata.

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Abe, S., Obata, Y., Oka, S. et al. Chondroitin sulfate prevents peritoneal fibrosis in mice by suppressing NF-κB activation. Med Mol Morphol 49, 144–153 (2016). https://doi.org/10.1007/s00795-016-0133-8

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  • DOI: https://doi.org/10.1007/s00795-016-0133-8

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