Extremophiles

, Volume 5, Issue 2, pp 85–91

Isolation and characterization of Thermococcus sibiricus sp. nov. from a Western Siberia high-temperature oil reservoir

  • Margarita L. Miroshnichenko
  • Hans Hippe
  • Erko Stackebrandt
  • Nadezhda A. Kostrikina
  • Nikolai A. Chernyh
  • Christian Jeanthon
  • Tamara N. Nazina
  • Sergei S. Belyaev
  • Elizaveta A. Bonch-Osmolovskaya
Original paper

DOI: 10.1007/s007920100175

Cite this article as:
Miroshnichenko, M.L., Hippe, H., Stackebrandt, E. et al. Extremophiles (2001) 5: 85. doi:10.1007/s007920100175

Abstract.

Anaerobic organotrophic hyperthermophilic Archaea were isolated from five of eight samples from oil wells of the Samotlor oil reservoir (depth, 1,799–2,287 m; temperature, 60°–84°C). Three strains were isolated in pure cultures and characterized phylogenetically on the basis of comparison of the 16S rRNA gene sequences. All strains belonged to a new species of the genus Thermococcus, with Thermococcus litoralis, Thermococcus aggregans, Thermococcus fumicolans, and Thermococcus alcaliphilus being the nearest relatives (range of sequence similarity, 97.2%–98.8%). Strain MM 739 was studied in detail. The new isolate grew on peptides but not on carbohydrates. Elemental sulfur had a stimulatory effect on growth. The temperature range for growth was between 40° and 88°C, with the optimum at 78°C; the pH range was 5.8 to 9.0, with the optimum around 7.3; and the salinity range was 0.5% to 7.0%, with the optimum at 1.8%–2.0%. The doubling time at optimal growth conditions was about 43 min. The G+C content of the DNA was 38.4 mol%. The DNA–DNA relatedness between strain MM 739 and T. litoralis was 27%; between strain MM 739 and T. aggregans, it was 22%. Based on the phenotypic and genomic differences with known Thermococcus species, the new species Thermococcus sibiricus is proposed. The isolation of a hyperthermophilic archaeum from a deep subsurface environment, significantly remote from shallow or abyssal marine hot vents, indicates the existence of a subterranean biosphere inhabited by indigenous hyperthermophilic biota.

Deep subsurface environment Hyperthermophilic Archaea Thermococcus sibiricus

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Margarita L. Miroshnichenko
    • 1
  • Hans Hippe
    • 2
  • Erko Stackebrandt
    • 2
  • Nadezhda A. Kostrikina
    • 1
  • Nikolai A. Chernyh
    • 1
  • Christian Jeanthon
    • 3
  • Tamara N. Nazina
    • 1
  • Sergei S. Belyaev
    • 1
  • Elizaveta A. Bonch-Osmolovskaya
    • 1
  1. 1.Institute of Microbiology, Russian Academy of Sciences, Prospect 60, Letiya Oktyabrya 7/2, 117811 Moscow, Russia
  2. 2.DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen) GmbH, Braunschweig, Germany
  3. 3.Station Biologique, Centre National de la Recherche Scientifique and Université Pierre et Marie Curie, Roscoff, France
  4. 4.Present address: Université de Bretagne Occidentale, Institut Universitaire Européen de la Mer, Plouzané, France