Extremophiles

, Volume 1, Issue 3, pp 117–123

Molecular analyses of the sediment of the 11000-m deep Mariana Trench

  • Chiaki Kato
  • Lina Li
  • Jin Tamaoka
  • Koki Horikoshi
ORIGINAL PAPER

DOI: 10.1007/s007920050024

Cite this article as:
Kato, C., Li, L., Tamaoka, J. et al. Extremophiles (1997) 1: 117. doi:10.1007/s007920050024

Abstract

We have obtained sediment samples from the world's deepest sea-bottom, the Mariana Trench challenger point at a depth of 10 898 m, using the new unmanned submersible Kaiko. DNA was extracted from the sediment, and DNA fragments encoding several prokaryotic ribosomal RNA small-subunit sequences and pressure-regulated gene clusters, typically identifed in deep-sea adapted bacteria, were amplifed by the polymerase chain reaction. From the sequencing results, at least two kinds of bacterial 16S rRNAs closely related to those of the genus Pseudomonas and deep-sea adapted marine bacteria, and archaeal 16S rRNAs related to that of a planktonic marine archaeon were identifed. The sequences of the amplifed pressure-regulated clusters were more similar to those of deep-sea barophilic bacteria than those of barotolerant bacteria. These results suggest that deep-sea adapted barophilic bacteria, planktonic marine archaea, and some of the world's most widespread bacteria (the genus Pseudomonas) coexist on the world's deepest sea-bottom.

Key words Mariana TrenchChallenger pointDeep-seaBarophilic bacteriaPseudomonasPlanktonic marine archaeaPressure-regulated operon

Copyright information

© Springer-Verlag Tokyo 1997

Authors and Affiliations

  • Chiaki Kato
    • 1
  • Lina Li
    • 1
  • Jin Tamaoka
    • 1
  • Koki Horikoshi
    • 1
  1. 1.The Deepstar Group, Japan Marine Science and Technology Center (JAMSTEC), 2-15 Natsushima-cho, Yokosuka 237, Japan Tel. +81-468-67-5555; Fax +81-468-66-6364 ¶e-mail: katoc@jamstec.go.jpJP