Abstract
Permafrost on the Qinghai-Tibet Plateau is one of the most sensitive regions to climate warming, thus characterizing its microbial diversity and community composition may be important for understanding their potential responses to climate changes. Here, we investigated the prokaryotic diversity in a 10-m-long permafrost core from the Qinghai-Tibet Plateau by restriction fragment length polymorphism analysis targeting the 16S rRNA gene. We detected 191 and 17 bacterial and archaeal phylotypes representing 14 and 2 distinct phyla, respectively. Proteobacteria was the dominant bacterial phylum, while archaeal communities were characterized by a preponderance of Thaumarchaeota. Some of prokaryotic phylotypes were closely related to characterized species involved in carbon and nitrogen cycles, including nitrogen fixation, methane oxidation and nitrification. However, the majority of the phylotypes were only distantly related to known taxa at order or species level, suggesting the potential of novel diversity. Additionally, both bacterial α diversity and community composition changed significantly with sampling depth, where these communities mainly distributed according to core horizons. Arthrobacter-related phylotypes presented at high relative abundance in two active layer soils, while the deeper permafrost soils were dominated by Psychrobacter-related clones. Changes in bacterial community composition were correlated with most measured soil variables, such as carbon and nitrogen contents, pH, and conductivity.
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Acknowledgments
We are grateful to other members from Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences for successful field work. This research was supported by funding from the National Basic Research Program (2012CB026105), National Natural Science Foundation (31170482, 31300445, 31370450), PhD Programs Foundation of Ministry of Education (20130211120005), the Chinese Postdoctoral Science Foundation (2013M540780, 2014T70949), Fundamental Research Funds for the Central Universities in China (LZUJBKY-2011-119) and State Key Laboratory of Frozen Soil Engineering, Chinese Academy of Sciences (SKLFSE200901).
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The soil samples used in this study were collected in the state-owned land which is open for scientific research. No specified permissions are required for this sampling site, which is not natural reserve and did not involve endangered or protected species.
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W. Hu and Q. Zhang contributed equally to this work.
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Fig. S3 Phylogenetic analysis of bacterial phylotypes grouping with the Actinobacteria, Firmicutes and an unclassified phylum (DOCX 123 kb)
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Hu, W., Zhang, Q., Tian, T. et al. Characterization of the prokaryotic diversity through a stratigraphic permafrost core profile from the Qinghai-Tibet Plateau. Extremophiles 20, 337–349 (2016). https://doi.org/10.1007/s00792-016-0825-y
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DOI: https://doi.org/10.1007/s00792-016-0825-y