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Characterization of Alkaliphilus hydrothermalis sp. nov., a novel alkaliphilic anaerobic bacterium, isolated from a carbonaceous chimney of the Prony hydrothermal field, New Caledonia

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Abstract

A novel anaerobic, alkaliphilic, Gram-positive staining bacterium was isolated from a hydrothermal chimney in the Prony Bay, New Caledonia. This strain designated FatMR1T grew at temperatures from 20 to 55 °C (optimum 37 °C) and at pH between 7.5 and 10.5 (optimum 8.8–9). NaCl is not required for growth (optimum 0.2–0.5 %), but is tolerated up to 3 %. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite are not used as terminal electron acceptors. Strain FatMR1T fermented pyruvate, yeast extract, peptone and biotrypcase and used fructose as the only sugar. The main fermentation products from fructose and proteinaceous compounds (e.g. peptone and biotrypcase) were acetate, H2 and CO2. Crotonate was disproportionated to acetate and butyrate. The predominant cellular fatty acids were C14:0 and C16:0. The G + C content of the genomic DNA was 37.1 mol %. On the basis of phylogenetic, genetic, and physiological properties, strain FatMR1T (=DSM 25890T, =JCM 18390T) belonging to the phylum Firmicutes, class Clostridia, order Clostridiales, is proposed as a novel species of the genus Alkaliphilus, A. hydrothermalis sp. nov.

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Acknowledgments

We would like to thank Manon Joseph for electron microscopy photographs. We thank Régis Hocdé and Jean-Louis Menou for sampling by scubadiving in Prony Bay.

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Authors and Affiliations

  1. IRD, Mediterranean Institute of Oceanography (MIO), CNRS/INSU, Aix-Marseille Université, Université du Sud Toulon-Var, UM 110, 13288, Marseille Cedex 09, France

    Fatma Ben Aissa, Anne Postec, Gaël Erauso, Marie-Laure Fardeau & Bernard Ollivier

  2. Laboratoire d’Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080, Tunis Cedex, Tunisia

    Fatma Ben Aissa & Moktar Hamdi

  3. Institut pour la Recherche et le Développement (IRD) Centre de Nouméa, Nouméa, 98848, Nouvelle Calédonie

    Claude Payri & Bernard Pelletier

Authors
  1. Fatma Ben Aissa
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  2. Anne Postec
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  3. Gaël Erauso
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  4. Claude Payri
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  5. Bernard Pelletier
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  6. Moktar Hamdi
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  7. Marie-Laure Fardeau
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  8. Bernard Ollivier
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Corresponding author

Correspondence to Bernard Ollivier.

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Communicated by A. Oren.

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Ben Aissa, F., Postec, A., Erauso, G. et al. Characterization of Alkaliphilus hydrothermalis sp. nov., a novel alkaliphilic anaerobic bacterium, isolated from a carbonaceous chimney of the Prony hydrothermal field, New Caledonia. Extremophiles 19, 183–188 (2015). https://doi.org/10.1007/s00792-014-0697-y

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  • DOI: https://doi.org/10.1007/s00792-014-0697-y

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