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Deletion of cdvB paralogous genes of Sulfolobus acidocaldarius impairs cell division

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Abstract

The majority of Crenarchaeota utilize the cell division system (Cdv) to divide. This system consists of three highly conserved genes, cdvA, cdvB and cdvC that are organized in an operon. CdvC is homologous to the AAA-type ATPase Vps4, involved in multivesicular body biogenesis in eukaryotes. CdvA is a unique archaeal protein that interacts with the membrane, while CdvB is homologous to the eukaryal Vps24 and forms helical filaments. Most Crenarcheota contain additional CdvB paralogs. In Sulfolobus acidocaldarius these are termed CdvB1–3. We have used a gene inactivation approach to determine the impact of these additional cdvB genes on cell division. Independent deletion mutants of these genes were analyzed for growth and protein localization. One of the deletion strains (ΔcdvB3) showed a severe growth defect on plates and delayed growth on liquid medium. It showed the formation of enlarged cells and a defect in DNA segregation. Since these defects are accompanied with an aberrant localization of CdvA and CdvB, we conclude that CdvB3 fulfills an important accessory role in cell division.

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Acknowledgments

Thanks to Dirk-Jan Scheffers for help with the fluorescence microscopy, and Maarten Mols for assistance in the flow cytometry. We are grateful to Ralf Bernander (Stockholm University, Sweden) for kindly providing the Cdv antibodies.

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Authors and Affiliations

  1. Department of Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, and the Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 7, 9747 AG, Groningen, The Netherlands

    Nuan Yang & Arnold J. M. Driessen

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  1. Nuan Yang
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  2. Arnold J. M. Driessen
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Correspondence to Arnold J. M. Driessen.

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Communicated by L. Huang.

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Yang, N., Driessen, A.J.M. Deletion of cdvB paralogous genes of Sulfolobus acidocaldarius impairs cell division. Extremophiles 18, 331–339 (2014). https://doi.org/10.1007/s00792-013-0618-5

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  • DOI: https://doi.org/10.1007/s00792-013-0618-5

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