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Effects of intracellular Mn on the radiation resistance of the halophilic archaeon Halobacterium salinarum

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Abstract

Ionizing radiation (IR) is of particular interest in biology because its exposure results in severe oxidative stress to the cell’s macromolecules. Our recent work with extremophiles supports the idea that IR resistance is most likely achieved by a metabolic route, effected by manganese (Mn) antioxidants. Biochemical analysis of “super-IR resistant” mutants of H. salinarum, evolved over multiple cycles of exposure to high doses of IR, confirmed the key role for Mn antioxidants in the IR resistance of this organism. Analysis of the proteome of H. salinarum “super-IR resistant” mutants revealed increased expression for proteins involved in energy metabolism, replenishing the cell with reducing equivalents depleted by the oxidative stress inflicted by IR. Maintenance of redox homeostasis was also activated by the over-expression of coenzyme biosynthesis pathways involved in redox reactions. We propose that in H. salinarum, increased tolerance to IR is a combination of metabolic regulatory adjustments and the accumulation of Mn-antioxidant complexes.

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Acknowledgments

This work was supported by the AFOSR (Grant FA95500710158) to J. DiRuggiero. The authors thank Elena Gaidamakova and Vera Matrosova at the Uniformed Services University of the Health Sciences (USUHS) for their technical support using the gamma source at USUHS and M. J. Daly for his support.

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  1. Department of Biology, Johns Hopkins University, 3400 N. Charles St, Mudd Hall, Baltimore, MD, 21218, USA

    Kimberly M. Webb, Jerry Yu, Courtney K. Robinson, Tomiya Noboru, Yuan C. Lee & Jocelyne DiRuggiero

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  1. Kimberly M. Webb
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  2. Jerry Yu
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  3. Courtney K. Robinson
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  4. Tomiya Noboru
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Correspondence to Jocelyne DiRuggiero.

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Communicated by F. Robb.

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Webb, K.M., Yu, J., Robinson, C.K. et al. Effects of intracellular Mn on the radiation resistance of the halophilic archaeon Halobacterium salinarum . Extremophiles 17, 485–497 (2013). https://doi.org/10.1007/s00792-013-0533-9

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