Original Paper

Extremophiles

, Volume 12, Issue 1, pp 119-124

Open Access This content is freely available online to anyone, anywhere at any time.

Tetraether membrane lipids of Candidatus “Aciduliprofundum boonei”, a cultivated obligate thermoacidophilic euryarchaeote from deep-sea hydrothermal vents

  • Stefan SchoutenAffiliated withDepartment of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research Email author 
  • , Marianne BaasAffiliated withDepartment of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research
  • , Ellen C. HopmansAffiliated withDepartment of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research
  • , Anna-Louise ReysenbachAffiliated withDepartment of Biology, Portland State University
  • , Jaap S. Sinninghe DamstéAffiliated withDepartment of Marine Biogeochemistry and Toxicology, Royal Netherlands Institute for Sea Research

Abstract

The lipid composition of Candidatus “Aciduliprofundum boonei”, the only cultivated representative of archaea falling in the DHVE2 phylogenetic cluster, a group of microorganisms ubiquitously occurring at hydrothermal vents, was studied. The predominant core membrane lipids in this thermophilic euryarchaeote were found to be composed of glycerol dibiphytanyl glycerol tetraethers (GDGTs) containing 0–4 cyclopentyl moieties. In addition, GDGTs with an additional covalent bond between the isoprenoid hydrocarbon chains, so-called H-shaped GDGTs, were present. The latter core lipids have been rarely reported previously. Intact polar lipid analysis revealed that they predominantly consist of GDGTs with a phospho-glycerol headgroup.

Keywords

Aciduliprofundum boonei DHVE2 cluster Glycerol dialkyl glycerol tetraethers Thermoacidophile