Abstract
Objective
The aim of the study is to compare detection frequency of periodontal pathogens in patients with aggressive/severe chronic periodontitis using pooled plaque samples from the deepest pockets per quadrant/per sextant.
Methods
In 100 patients with aggressive/chronic periodontitis, subgingival plaque was sampled from the deepest pockets per quadrant (MT4) and per sextant (MT6). Plaque samples were taken using two sterile paper points simultaneously. One paper point from each pocket was pooled with the three other paper points of the pockets (MT4). Subsequently, the remaining four paper points were pooled with two paper points from the deepest pockets from the two remaining sextants (MT6). The content of each vial was analyzed with nucleic-acid based methods for Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Porphyromonas gingivalis, Treponema denticola, Prevotella intermedia, Parvimonas micra, Fusobacterium nucleatum, Campylobacter rectus, Eubacterium nodatum, Eikenella corrodens, and Capnocytophaga sp.
Results
The detection frequency of A. actinomycetemcomitans (MT4/MT6) at 22/24 %, T. forsythia at 93/96 %, P. gingivalis at 78/79 %, T. denticola at 88/90 %, P. intermedia at 40/46 %, P. micra at 75/79 %, F. nucleatum at both 99 %, C. rectus at 84/89 %, E. nodatum at 62/65 %, E. corrodens at 80/87 %, and Capnocytophaga sp. at 49/58 % was higher with MT6 than with MT4. None of these differences were statistically significant.
Conclusion
The detection frequency of the investigated periopathogens was statistically insignificant higher with the sampling method MT6 compared with MT4.
Clinical relevance
In daily dental practice, the plaque sampling of the deepest pockets per quadrant seems to be sufficient.
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References
Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, Sahasrabudhe A, Dewhirst FE (2001) Bacterial diversity in human subgingival plaque. J Bacteriol 183:3770–3783
Paster BJ, Olsen I, Aas JA, Dewhirst FE (2006) The breadth of bacterial diversity in the human periodontal pocket and other oral sites. Periodontol 2000 42:80–87
Nørskov-Lauritsen N, Kilian M (2006) Reclassification of Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, Haemophilus paraphrophilus and Haemophilus segnis as Aggregatibacter actinomycetemcomitans gen. nov., comb. nov., Aggregatibacter aphrophilus comb. nov. and Aggregatibacter segnis comb. nov., and emended description of Aggregatibacter aphrophilus to include V factor-dependent and V factor-independent isolates. Int J Syst Evol Microbiol 56:2135–2146
Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr (1998) Microbial complexes in subgingival plaque. J Clin Periodontol 25:134–144
van Winkelhoff AJ, Loos BG, van der Reijden WA, van der Velden U (2002) Porphyromonas gingivalis and Bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction. J Clin Periodontol 29:1023–1028
Bragd L, Dahlen G, Wikström M, Slots J (1985) The capability of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius to indicate progressive periodontitis; retrospective study. J Clin Periodontol 14:95–99
Newman MG, Socransky SS, Savitt ED, Propas DA, Crawford A (1976) Studies of the microbiology of periodontosis. J Periodontol 47:373–379
Tonetti MS, Mombelli A (1999) Early-onset periodontitis. Ann Periodontol 4:39–52
Schacher B, Baron F, Rossberg M, Wohlfeil M, Arndt R, Eickholz P (2007) Aggregatibacter actinomycetemcomitans as indicator for aggressive periodontitis by two analysing strategies. J Clin Periodontol 34:566–573
Haubek D, Ennibi O-K, Væth M, Poulsen S, Kilian M (2008) Risk of aggressive periodontitis in adolescent carriers of the JP2 clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans in Morocco: a prospective longitudinal cohort study. Lancet 371:237–242
Schacher B, Baron F, Ludwig B, Valesky E, Noack B, Eickholz P (2006) Periodontal therapy in siblings with Papillon-Lefèvre syndrome and tinea capitis: a report of two cases. J Clin Periodontol 33:829–836
Christersson L, Fransson C, Dunford R, Zambon J (1992) Microbiological and clinical effects of surgical treatment of localized juvenile periodontitis. J Peridontol 63:465–476
Kornman K, Robertson P (1985) Clinical and microbiological evaluation of therapy of juvenile peridontitis. J Periodontol 56:443–446
Müller H-P, Lange DE, Müller RF (1993) Failure of adjunctive minocycline-HCl to eliminate oral Actinobacillus actinomycetemcomitans. J Clin Periodontol 20:498–504
Mombelli A, Gmür R, Gobbi C, Lang NP (1994) Actinobacillus actinomycetemcomitans in adult periodontitis. I. Topographic distribution before and after treatment. J Periodontol 65:820–826
Takamatsu N, Yano K, He T, Umeda M, Ishikawa I (1999) Effect of initial periodontal therapy on the frequency of detecting Bacteroides forsythus, Porphyromonas gingivalis, and Actinobacillus actinomycetemcomitans. J Periodontol 70:574–580
Ehmke B, Moter A, Beikler T, Milian E, Flemmig TF (2005) Adjunctive antimicrobial therapy of periodontitis: long-term effects on disease progression and oral colonization. J Periodontol 76:749–759
American Academy of Periodontology (2000) Parameter on progressive periodontitis. J Periodontol 71:867–869
American Academy of Periodontology (2001) Treatment of plaque-induced gingivitis, chronic periodontitis, and other clinical conditions. J Periodontol 72:1790–1800
Beikler T, Prior K, Ehmke B, Flemmig TF (2004) Specific antibiotics in the treatment of periodontitis - A proposed strategy. J Periodontol 75:169–175
Beikler T, Karch H, Flemmig TF (2005) In German (Microbiologic diagnosis in periodontal therapy). Joint statement of the German Society of Periodontology (DGP) and the German Society of Dental, Oral, and Maxillofacial Medicine (DGZMK). Dtsch Zahnärztl Z 60:660–662
Krigar D-M, Kaltschmitt J, Krieger JK, Eickholz P (2007) Two subgingival plaque sampling strategies used with RNA-probes. J Periodontol 78:72–78
Baron F, Arndt R, Roßberg M, Schacher B, Wohlfeil M, Eickholz P (2008) In German (Prävalenz von Fusobacterium nucleatum und Prevotella intermedia in subgingivaler Plaque bei 2 Analysestrategien). Parodontologie 19:233–240
Beikler T, Schnitzer S, Abdeen G, Ehmke B, Eisenacher M, Flemmig TF (2006) Sampling strategy for intraoral detection of periodontal pathogenes before and following periodontal therapy. J Periodontol 77:1323–1332
Kim C-K, Choi S-H, Kim T-S, Kaltschmitt J, Eickholz P (2006) The infrabony defect and its determinants. J Periodontal Res 41:498–502
Fleiss JL (1981) Statistical methods for rates and proportions, 2nd edn. Wiley, New York, pp 223–225
van Winkelhoff AJ, Rodenburg JP, Goené RJ, Abbas EG, Winkel EG, de Graaff J (1989) Metronidazole plus amoxicillin in the treatment of Actinobacillus actinomycetemcomitans associated periodontitis. J Clin Periodontol 16:128–131
Dannewitz B, Pohl S, Eickholz P, Kim T-S (2007) Clinical and microbiological effects of a combined mechanic-antibiotic therapy in patients with Actinobacillus actinomycetemcomitans-associated periodontitis. Am J Dent 20:153–156
Eickholz P, Dannewitz B, Kim T-S (2005) Antibiotics in periodontal therapy. Perio 2:235–251
Ramich T, Schacher B, Scharf S, Röllke L, Arndt R, Eickholz P, Nickles K (2015) Subgingival plaque sampling after combined mechanical and antibiotic nonsurgical periodontal therapy. Clin Oral Investig 19:27–34
Eick S, Pfister W (2002) Comparison of microbial cultivation and a commercial PCR based method for detection of periodontopathogenetic species in subgingival plaque samples. J Clin Periodontol 29:638–644
Jervøe-Storm P-M, Koltzscher M, Falk W, Dörfler A, Jepsen S (2005) Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples. J Clin Periodontol 32:778–783
Mombelli A, McNabb H, Lang NP (1991) Black-pigmenting gram-negative bacteria in periodontal disease. I. Topographic distribution in the human dentition. J Periodontal Res 26:301–307
Jervøe-Storm PM, AlAhdab H, Koltzscher M, Fimmers R, Jepsen S (2010) Quantification of periodontal pathogens by paper point sampling from the coronal and apical aspect of periodontal lesions by real-time PCR. Clin Oral Investig 14:533–541
Jervøe-Storm P-M, AlAhdab H, Koltzscher M, Fimmers R, Jepsen S (2007) Comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction. J Periodontol 78:909–917
Sixou M, Duffaut-Lagarrigue D, Lodter JP (1991) In French (A comparison between 4 different subgingival bacteriologic sampling technics). J Biol Buccale 19:16–21
Renvert S, Wikström M, Helmersson M, Dahlén G, Claffey N (1992) Comparative study of subgingival microbiological sampling techniques. J Periodontol 63:797–801
Hartroth B, Seyfahrt I, Conrads G (1999) Sampling of periodontal pathogens by paper points: evaluation of basic parameters. Oral Microbiol Immunol 14:326–330
Haffajee AD, Socransky SS (1992) Effect of sampling strategy on the false-negative rate for detection of selected subgingival species. Oral Microbiol Immunol 7:57–59
Casas A, Herrera D, Martin-Carnes J, Gonzáles I, O’Connor A, Sanz M (2007) Influence of sampling strategy on microbiologic results before and after periodontal therapy. J Periodontol 78:1103–1112
Wohlfeil M, Tabakci O, Arndt R, Eickholz P, Nickles K (2010) Detection rates of presumptive periodontal pathogens in subgingival plaque samples of untreated periodontitis using either four or six pooled samples. J Investig Clin Dent 1:126–132
Mombelli A, Casagni F, Madianos PN (2002) Can presence or absence of periodontal pathogens distinguish between subjects with chronic and aggressive periodontitis? A systematic review. J Clin Periodontol 29(suppl 3):10–21
Gatto MR, Montevecchi M, Paolucci M, Landini MP, Checchi L (2014) Prevalence of six periodontal pathogens in subgingival samples of Italian patients with chronic periodontitis. New Microbiol 37:517–524
Acknowledgments
This study was supported by Hain Lifescience GmbH, Nehren, Germany.
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The authors report no conflicts of interest related to this study.
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Nickles, K., Scharf, S., Röllke, L. et al. Detection of subgingival periodontal pathogens—comparison of two sampling strategies. Clin Oral Invest 20, 571–579 (2016). https://doi.org/10.1007/s00784-015-1530-4
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DOI: https://doi.org/10.1007/s00784-015-1530-4