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Comparison of gene expression between mandibular and iliac bone-derived cells

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Abstract

Objectives

The purpose of this study is to investigate the differences in gene expression between the human mandibular and iliac bone-derived cells (BCs) for better understanding of the site-specific characteristics of bones.

Methods

Primary cells were obtained from mandibular and iliac bones from six healthy, elderly donors. To investigate site-specific differences, gene expression profile of mandibular and iliac BC from the same donors were compared via cDNA microarray analysis.

Results

A comparison of the gene expression profiles revealed that 82 genes were significantly upregulated and 66 genes were downregulated with 1.5 fold or greater in mandibular versus iliac BCs. The most significantly differentially regulated genes were associated with skeletal system development or morphogenesis (SIX1, MSX1, MSX2, HAND2, PRRX1, OSR2, HOX gene family, PITX2). Especially, upregulated genes in mandibular BC were related with tooth morphogenesis, originated from the ectomesenchyme. Microarray analysis revealed that Msx1 was 2.03-fold and Msx2 was 1.99-fold upregulated in mandibular versus iliac BCs (both p < 0.01). Furthermore, in mandibular BCs, all members of the HOX gene family that were analyzed were downregulated (p < 0.01) and osteopontin was also downregulated by 2.84-fold (p < 0.01).

Conclusions

Site-specific differences between jaw and long bones can be explained by the differences in gene expression patterns. Our results suggest that bone cell-derived cells maintain the genetic characteristics of their embryological origin.

Clinical relevance

This study revealed fundamental differences in gene expression between the mandibular and iliac bone in humans. These differences could be important for understanding jaw bone-specific development of bisphosphonate-related osteonecrosis of the jaw.

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Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF 2010-0011-445).

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Oral & Maxillofacial Surgery, School of Dentistry, Kyungpook National University, Daegu, Republic of Korea

    Jung-Tae Lee, So-Young Choi, Hyung-Lak Kim & Tae-Geon Kwon

  2. Department of Oral Biochemistry, School of Dentistry, Institute for Hard Tissue and Bio-Tooth Regeneration, Kyungpook National University, Daegu, Republic of Korea

    Jae-Young Kim

  3. Department of Oral Microbiology, School of Dentistry, Kyungpook National University, Samduck 2 Ga, Jung Gu, Daegu, 700-412, Republic of Korea

    Heon-Jin Lee

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  1. Jung-Tae Lee
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Correspondence to Tae-Geon Kwon.

Additional information

Jung-Tae Lee and So-Young Choi contributed equally to this paper.

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Lee, JT., Choi, SY., Kim, HL. et al. Comparison of gene expression between mandibular and iliac bone-derived cells. Clin Oral Invest 19, 1223–1233 (2015). https://doi.org/10.1007/s00784-014-1353-8

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  • DOI: https://doi.org/10.1007/s00784-014-1353-8

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