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Connective tissue graft as a biological barrier for guided tissue regeneration in intrabony defects: a histological study in dogs

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Abstract

Background

The use of the autogenous periosteal graft as biological barrier has been proposed for periodontal regeneration. The aim of this study was to evaluate the histometric findings of the subepithelial connective tissue graft as barrier in intrabony defects compared to a bioabsorbable membrane.

Methods

Three-walled intrabony defects were created surgically in the mesial aspect of the right and left maxillary canines in five healthy mongrel dogs. The defects were chronified, and two types of barriers were randomly carried out for guided tissue regeneration in a split-mouth design: the test group with a subepithelial connective tissue graft and the control group with a bioabsorbable membrane. The specimens were processed for histometric analyses of the epithelium (E), connective tissue (CT), newly formed cementum (NC), new bone (NB), and total newly formed tissues (NFT).

Results

The test side showed smaller mean of NC (3.6 ± 1.2), NB (2.1 ± 0.7), and NFT (7.7 ± 0.8) than the control group (NC 7.3 ± 0.5; NB 5.3 ± 1.3; NFT 10.1 ± 2.2; P < 0.05). No statistically significant differences were verified for E (test 3.1 ± 2.0; control 2.8 ± 2.1; P > 0.05) and CT (test 2.5 ± 1.1; control 2.0 ± 0.5; P > 0.05) between groups.

Conclusion

The bioabsorbable membrane was more effective in maintaining the space for periodontal regeneration than periosteal connective graft when used as barrier.

Clinical relevance

The bioabsorbable membrane showed more favorable regenerative results in intrabony defects in dogs than the subepithelial connective tissue graft as biological barrier.

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Acknowledgments

The authors would like to thank FAPESP (São Paulo Foundation for the Support of Research, grant no. 03/12810-5) for financial support.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Master in Dental Sciences, School of Dentistry, Educational Foundation of Barretos (UNIFEB), Av. Prof. Roberto Frade Monte, 389. Jd. Aeroporto, 14783-226, Barretos, SP, Brazil

    Fernando Salimon Ribeiro, Ana Emília Farias Pontes, Elizangela Partata Zuza & Raphael Carlos Comelli Lia

  2. Department of Dentistry II, School of Dentistry, Federal University of Maranhão (UFMA), Maranhão, MA, Brazil

    Vanessa Camila da Silva

  3. Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil

    Elcio Marcantonio Junior

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  1. Fernando Salimon Ribeiro
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  2. Ana Emília Farias Pontes
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  4. Vanessa Camila da Silva
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Correspondence to Fernando Salimon Ribeiro.

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Ribeiro, F.S., Pontes, A.E.F., Zuza, E.P. et al. Connective tissue graft as a biological barrier for guided tissue regeneration in intrabony defects: a histological study in dogs. Clin Oral Invest 19, 997–1004 (2015). https://doi.org/10.1007/s00784-014-1323-1

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  • DOI: https://doi.org/10.1007/s00784-014-1323-1

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