JBIC Journal of Biological Inorganic Chemistry

, Volume 16, Issue 4, pp 621–632

A novel zinc bis(thiosemicarbazone) complex for live cell imaging

  • Disha Dayal
  • Duraippandi Palanimuthu
  • Sridevi Vijay Shinde
  • Kumaravel Somasundaram
  • Ashoka G. Samuelson
Original Paper

DOI: 10.1007/s00775-011-0764-0

Cite this article as:
Dayal, D., Palanimuthu, D., Shinde, S.V. et al. J Biol Inorg Chem (2011) 16: 621. doi:10.1007/s00775-011-0764-0

Abstract

Fluorescent zinc complexes have recently attracted a lot of interest owing to their vast applications in cellular imaging. We report the synthesis as well as physical, chemical and biological studies of a novel zinc glyoxalbis(4-methyl-4-phenyl-3-thiosemicarbazone), [Zn(GTSC)]3, complex. As compared with the well-studied zinc biacetylbis(4-methyl-3-thiosemicarbazone), Zn(ATSM), complex, which was used as a reference, [Zn(GTSC)]3 had 2.5-fold higher fluorescence. When cellular fluorescence was measured using flow cytometry, we observed that [Zn(GTSC)]3 had 3.4-fold to 12-fold higher fluorescence than Zn(ATSM) in various cell lines (n = 9) of different tissue origin. Confocal fluorescence microscopy results showed that [Zn(GTSC)]3 appeared to have a nuclear localization within 30 min of addition to MCF7 cells. Moreover, [Zn(GTSC)]3 showed minimal cytotoxicity compared with Zn(ATSM), suggesting that [Zn(GTSC)]3 may be less deleterious to cells when used as an imaging agent. Our data suggest that the novel [Zn(GTSC)]3 complex can potentially serve as a biocompatible fluorescent imaging agent for live cells.

Keywords

Bis(thiosemicarbazone)Imaging agentCell viabilityBreast cancerZinc

Supplementary material

775_2011_764_MOESM1_ESM.pdf (429 kb)
Supporting Information (PDF 429 kb)

Copyright information

© SBIC 2011

Authors and Affiliations

  • Disha Dayal
    • 1
  • Duraippandi Palanimuthu
    • 2
  • Sridevi Vijay Shinde
    • 1
  • Kumaravel Somasundaram
    • 1
  • Ashoka G. Samuelson
    • 2
  1. 1.Department of Microbiology and Cell BiologyIndian Institute of ScienceBangaloreIndia
  2. 2.Department of Inorganic and Physical ChemistryIndian Institute of ScienceBangaloreIndia