Amino Acids

, Volume 46, Issue 3, pp 499–509

Innovative approaches to the use of polyamines for DNA nanoparticle preparation for gene therapy

Authors

  • Veena Vijayanathan
    • Departments of MedicineRutgers Robert Wood Johnson Medical School
    • Albert Einstein College of MedicineYeshiva University
  • Enzo Agostinelli
    • Istituto Pasteur-Fondazione Cenci Bolognetti, Department of Biochemical Sciences “A. Rossi Fanelli”SAPIENZA University of Rome and CNR, Institute of Biology and Molecular Pathology
  • Thresia Thomas
    • Departments of MedicineRutgers Robert Wood Johnson Medical School
    • Environmental and Community MedicineRutgers Robert Wood Johnson Medical School
    • Departments of MedicineRutgers Robert Wood Johnson Medical School
Minireview Article

DOI: 10.1007/s00726-013-1549-2

Cite this article as:
Vijayanathan, V., Agostinelli, E., Thomas, T. et al. Amino Acids (2014) 46: 499. doi:10.1007/s00726-013-1549-2

Abstract

Advances in genomic technologies, such as next generation sequencing and disease specific gene targeting through anti-sense, anti-gene, siRNA and microRNA approaches require the transport of nucleic acid drugs through the cell membrane. Membrane transport of DNA/RNA drugs is an inefficient process, and the mechanism(s) by which this process occurs is not clear. A pre-requisite for effective transport of DNA and RNA in cells is their condensation to nanoparticles of ~100 nm size. Although viral vectors are effective in gene therapy, the immune response elicited by viral proteins poses a major challenge. Multivalent cations, such as natural polyamines are excellent promoters of DNA/RNA condensation to nanoparticles. During the past 20 years, our laboratory has synthesized and tested several analogs of the natural polyamine, spermine, for their efficacy to provoke DNA condensation to nanoparticles. We determined the thermodynamics of polyamine-mediated DNA condensation, measured the structural specificity effects of polyamine analogs in facilitating the cellular uptake of oligonucleotides, and evaluated the gene silencing activity of DNA nanoparticles in breast cancer cells. Polyamine-complexed oligonucleotides showed a synergistic effect on target gene inhibition at the mRNA level compared to the use of polyamines and oligonucleotides as single agents. Ionic and structural specificity effects were evident in DNA condensation and cellular transportation effects of polyamines. In condensed DNA structures, correlation exists between the attractive and repulsive forces with structurally different polyamines and cobalt hexamine, indicating the existence of a common force in stabilizing the condensed structures. Future studies aimed at defining the mechanism(s) of DNA compaction and structural features of DNA nanoparticles might aid in the development of novel gene delivery vehicles.

Keywords

Polyamines Nanoparticles Gene therapy DNA condensation Gene delivery Reactive oxygen species

Copyright information

© Springer-Verlag Wien 2013