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The postprandial use of dietary amino acids as an energy substrate is delayed after the deamination process in rats adapted for 2 weeks to a high protein diet

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Abstract

The aim of this study was to determine the contribution of dietary amino acids (AA) to energy metabolism under high protein (HP) diets, using a double tracer method to follow simultaneously the metabolic fate of α-amino groups and carbon skeletons. Sixty-seven male Wistar rats were fed a normal (NP) or HP diet for 14 days. Fifteen of them were equipped with a permanent catheter. On day 15, after fasting overnight, they received a 4-g meal extrinsically labeled with a mixture of 20 U-[15N]-[13C] AA. Energy metabolism, dietary AA deamination and oxidation and their transfer to plasma glucose were measured kinetically for 4 h in the catheterized rats. The transfer of dietary AA to liver glycogen was determined at 4 h. The digestive kinetics of dietary AA, their transfer into liver AA and proteins and the liver glycogen content were measured in the 52 other rats that were killed sequentially hourly over a 4-h period. [15N] and [13C] kinetics in the splanchnic protein pools were perfectly similar. Deamination increased fivefold in HP rats compared to NP rats. In the latter, all deaminated AA were oxidized. In HP rats, the oxidation rate was slower than deamination, so that half of the deaminated AA was non-oxidized within 4 h. Non-oxidized carbon skeletons were poorly sequestrated in glycogen, although there was a significant postprandial production of hepatic glycogen. Our results strongly suggest that excess dietary AA-derived carbon skeletons above the ATP production capacity, are temporarily retained in intermediate metabolic pools until the oxidative capacities of the liver are no longer overwhelmed by an excess of substrates.

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Acknowledgments

We are indebted to the foundation Le Lous for their financial support. We thank Sophie Daré for her help in animal catheterization and in indirect calorimetry experiments.

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Authors and Affiliations

  1. Usc914 Nutrition Physiology and Ingestive Behavior, INRA, CRNH-IdF, 75005, Paris, France

    Claire Fromentin, Dalila Azzout-Marniche, Daniel Tomé, Patrick Even, Catherine Luengo, Julien Piedcoq, Gilles Fromentin & Claire Gaudichon

  2. Usc914 Nutrition Physiology and Ingestive Behavior, AgroParisTech, CRNH-IdF, 75005, Paris, France

    Claire Fromentin, Dalila Azzout-Marniche, Daniel Tomé, Patrick Even, Catherine Luengo, Julien Piedcoq, Gilles Fromentin & Claire Gaudichon

  3. Usc914 Nutrition Physiology and Ingestive Behavior, AgroParisTech, 16 rue Claude Bernard, 75005, Paris, France

    Claire Gaudichon

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  1. Claire Fromentin
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  2. Dalila Azzout-Marniche
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Fromentin, C., Azzout-Marniche, D., Tomé, D. et al. The postprandial use of dietary amino acids as an energy substrate is delayed after the deamination process in rats adapted for 2 weeks to a high protein diet. Amino Acids 40, 1461–1472 (2011). https://doi.org/10.1007/s00726-010-0756-3

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