Summary.
We have examined the effects of midazolam, Ro 5-4864 (agonist for “peripheral” [p] benzodiazepine receptors [BR]), PK 11195 (antagonist for pBR), flumazenil (antagonist for “central” BR), naloxone (antagonist for opiate receptors) and the combination of midazolam and Ro 5-4864, PK 11195, flumazenil or naloxone on intracellular amino- and α-keto acids and the immune function markers superoxide anion (O2−), hydrogen peroxide (H2O2) and released myeloperoxidase (MPO) activity in neutrophils (PMN). Only midazolam and Ro 5-4864 led to significant changes in the dynamic PMN free amino- and α-keto acid pools. Concerning PMN immune function markers, midazolam and Ro 5-4864 significantly decreased O2− and H2O2 formation and released MPO. When midazolam and Ro 5-4864 were applied together they appeared to act additively. Pre-incubation with PK 11195 partially neutralized the midazolam effects whereas flumazenil or naloxone showed no effects. We therefore believe that pBR are involved in the signal transmission of anesthetic-induced cellular metabolic changes in PMN.
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Mühling, J., Gonter, J., Nickolaus, K. et al. Benzodiazepine receptor-dependent modulation of neutrophil (PMN) free amino- and α-keto acid profiles or immune functions. Amino Acids 28, 85–98 (2005). https://doi.org/10.1007/s00726-004-0136-y
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DOI: https://doi.org/10.1007/s00726-004-0136-y