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Genetic elaborations of glandular and non-glandular trichomes in Mentha arvensis genotypes: assessing genotypic and phenotypic correlations along with gene expressions

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Abstract

Mentha arvensis (corn mint) is well known for the production of menthol, a widely used commodity in pharma and flavoring industries and provides natural fragrances and products. Glandular trichomes are specialized hairs found on the aerial surface of vascular plants species producing specific secondary metabolite chemistry. Correlations were established among trichomes, oil yield, and major secondary metabolites. Nine improved, elite cultivars representing different M. arvensis genotypes were used for analysis. Phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) were estimated; results indicated the presence of considerable amount of genetic variability, thereby emphasizing wide scope of selection. Positive and significant associations were found among glandular trichomes, oil yield, essential oil constituents, and leaf morphology itself, whereas morphological parameters of leaf show positive and negative correlations to average number of trichome and essential oil constituents. Average number of glandular, non-glandular trichomes, their ratios, menthol content, and trichome number showed a good heritability. Trichomes were studied microscopically in leaf parts in all varieties for analyzing their distribution pattern. The trichome number variations showed significant correlation throughout the genotypes with essential oil yield and monoterpenoid constituents. Differential changes were analyzed for Glutathione S-transferases, Glutathione reductase, Malondialdehyde, phenolics, and chlorophyll content. Gene expressions were analyzed for biosynthesis genes and selected transcription factors TRANSPARENT TESTA GLABRA 1 (TTG1), ENOLASE 1, GLABRA 3, GTL 1, NUCLEAR TRANSCRIPTION FACTOR Y SUBUNIT B-6, WRKY transcription factor 22, putative WRKY 33, WRKY 17, WRKY 1, and WRKY 65-like for harnessing their relation with trichome development in M. arvensis genotypes.

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Acknowledgments

The authors express their sincere gratitude to the director of CSIR-CIMAP, India for keen interest and providing facilities for the experiments. Anand Mishra was supported by CSIR—Senior Research Fellowship, India. The authors gratefully acknowledge to USIC, B. B. Ambedkar University, India for providing the SEM facility.

Funding

This work was supported by the Department of Biotechnology, Bio-CARe award project grant BT/AB/08/01/2008(III) GAP 311 and Council of Scientific and Industrial Research, India (CSIR Network Project BSC0110).

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Authors and Affiliations

  1. Biotechnology Division, CSIR—Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India

    Anand Mishra

  2. Plant Breeding Division, CSIR—Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India

    R. K. Lal

  3. Analytical Chemistry Division, CSIR—Central Institute of Medicinal and Aromatic Plants, Lucknow, Uttar Pradesh, 226015, India

    C. S. Chanotiya

  4. Biotechnology Division, CSIR—Central Institute of Medicinal and Aromatic Plants, P.O. CIMAP, Lucknow, Uttar Pradesh, 226015, India

    Sunita Singh Dhawan

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  1. Anand Mishra
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Correspondence to Sunita Singh Dhawan.

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Mishra, A., Lal, R.K., Chanotiya, C.S. et al. Genetic elaborations of glandular and non-glandular trichomes in Mentha arvensis genotypes: assessing genotypic and phenotypic correlations along with gene expressions. Protoplasma 254, 1045–1061 (2017). https://doi.org/10.1007/s00709-016-1011-x

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