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Implication of long-distance cytoplasmic transport into dynamics of local pH on the surface of microinjured Chara cells

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Abstract

Cytoplasmic streaming is essential for intracellular communications but its specific functions are not well known. In Chara corallina internodes, long-distance interactions mediated by cyclosis are clearly evident with microscopy-pulse amplitude modulation (PAM) fluorometer under application of localized light (LL) pulses to a remote cell region. Measurements of LL-induced profiles of chlorophyll fluorescence F′ at various distances from the LL source suggest that illuminated chloroplasts release into the streaming cytoplasm excess reducing equivalents that are entrained by the fluid flow and transiently reduce the intersystem electron carriers in chloroplasts of downstream shaded areas. The reducing equivalents propagate to distances up to 4.5 mm from the LL source, with the transport rate nearly equal to the velocity of liquid flow. The F′ transients disappeared after the arrest of streaming with cytochalasin D and reappeared upon its recovery in washed cells. The F′ responses to a distant LL were used as an indicator for the passage of cytosolic reductants across the analyzed cell area during measurements of cell surface pH (pHo) in intact and microperforated internodes. In microwounded cell regions, the LL-induced increase in F′ occurred synchronously with the increase in pHo, by contrast to a slight decrease in pHo observed prior to perforation. The results show that reducing agents transported with the cytoplasmic flow are involved in rapid pH changes on the surface of microinjured cells. A possibility is considered that cytoplasmic reductants are processed by stress-activated plasmalemmal NADPH oxidase carrying electrons to oxygen with the eventual H+ consumption on the outer cell side.

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Abbreviations

AOI:

Area of inspection (area of interest)

CW:

Cell wall

LL:

Localized lighting (localized light)

PFD:

Photon flux density

pHo :

pH on the cell surface

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Acknowledgments

We are grateful to Prof. Ilse Foissner (University of Salzburg) for the gift of cytochalasin D and helpful discussion. This work was supported by the Russian Foundation for Basic Research, project no. 16-04-00318.

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Authors and Affiliations

  1. Faculty of Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    Alexander A. Bulychev & Anna V. Komarova

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  1. Alexander A. Bulychev
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  2. Anna V. Komarova
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Correspondence to Alexander A. Bulychev.

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Bulychev, A.A., Komarova, A.V. Implication of long-distance cytoplasmic transport into dynamics of local pH on the surface of microinjured Chara cells. Protoplasma 254, 557–567 (2017). https://doi.org/10.1007/s00709-016-0975-x

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  • DOI: https://doi.org/10.1007/s00709-016-0975-x

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