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Overexpression of AT14A confers tolerance to drought stress-induced oxidative damage in suspension cultured cells of Arabidopsis thaliana

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Abstract

Drought stress can affect interaction between plant cell plasma membrane and cell wall. Arabidopsis AT14A, an integrin-like protein, mediates the cell wall-plasma membrane-cytoskeleton continuum (WMC continuum). To gain further insight into the function of AT14A, the role of AT14A in response to drought stress simulated by polyethylene glycol (PEG-6000) in Arabidopsis suspension cultures was investigated. The expression of this gene was induced by PEG-6000 resulting from reverse transcription-PCR, which was further confirmed by the expression data from publically available microarray datasets. Compared to the wild-type cells, overexpression of AT14A (AT14A-OE) in Arabidopsis cultures exhibited a greater ability to adapt to water deficit, as evidenced by higher biomass accumulation and cell survival rate. Furthermore, AT14A-OE cells showed a higher tolerance to PEG-induced oxidative damage, as reflected by less H2O2 content, lipid peroxidation (malondialdehyde (MDA) content), and ion leakage, which was further verified by maintaining high levels of activities of antioxidant defense enzymes such as ascorbate peroxidase and guaiacol peroxidase and soluble protein. Taken together, our results suggest that overexpression of AT14A improves drought stress tolerance and that AT14A is involved in suppressing oxidative damage under drought stress in part via regulation of antioxidant enzyme activities

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 31101092; 31271622; 30800073), Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (Grant No.12KJD180008), Science and Technology Innovation Development Foundation of Yangzhou University (Grant No. 2013CXJ072), and the Priority Academic Program Development of Jiangsu Higher Education Institutions

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The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, 225009, China

    Lin Wang, Jie He, Haidong Ding, Hui Liu, Bing Lü, Jiansheng Liang, L. Wang, J. He, H. D. Ding, H. Liu, B. Lü & J. S. Liang

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  1. Lin Wang
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  2. Jie He
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  3. Haidong Ding
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  4. Hui Liu
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  5. Bing Lü
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  6. Jiansheng Liang
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  7. L. Wang
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  8. J. He
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  9. H. D. Ding
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  10. H. Liu
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  12. J. S. Liang
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Correspondence to J. S. Liang.

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Handling Editor: Bhumi Nath Tripathi

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Fig. S1

Expression profiles of AT14A induced by drought stress. Data was compiled from microarray data available using GENEVESTIGATOR tools (https://www.genevestigator.com/gv/plant.jsp). (A) Experiment ID AT-00560. (B) Experiment ID AT-00626. (3) Experiment ID AT-00657. (GIF 52 kb)

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Wang, L., He, J., Ding, H. et al. Overexpression of AT14A confers tolerance to drought stress-induced oxidative damage in suspension cultured cells of Arabidopsis thaliana . Protoplasma 252, 1111–1120 (2015). https://doi.org/10.1007/s00709-014-0744-7

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