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Subcellular localization of calcium in the incompatible and compatible interactions of wheat and Puccinia striiformis f. sp. tritici

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Abstract

Ca2+ is an ubiquitous intracellular molecule which is used as a second messenger to control many physiological activities in plant cells. In the present work, the relationship between calcium localization and the hypersensitive response (HR)—one of the most crucial and indispensable pathway to resist a pathogen—was studied in the wheat-wheat strip rust system using cytochemical technique. Our results show that calcium is involved in the interaction between wheat and wheat stripe rust. In the incompatible interaction associated with necrosis of host mesophyll cells, an influx of Ca2+ from the intercellular space to the cytoplasm and finally an efflux to the intercellular space again was detected in an incompatible interaction. Calcium precipitates were also observed in mesophyll cells adjacent to necrotic cells. On the contrary, calcium flow was not significantly altered in a compatible interaction. These results suggest that calcium might induce HR as a secondary messenger in the incompatible interaction of wheat and wheat stripe rust.

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Abbreviations

dpi:

Days post inoculation

EGTA:

Ethylene glycol tetraacetic acid

GT:

Germ tube

HC:

Host cells

HMC:

Haustorial mother cells

hpi:

Hours post inoculation

HR:

Hypersensitive response

IH:

Infection hyphae

IS:

Intercellular space

IT:

Infection type

NC:

Necrotic cells

PAMP:

Pathogen-associated molecular patterns

PR:

Pathogenesis-related

Pst :

Puccinia striiformis Westend f. sp. tritici Erikss.

PTI:

PAMP-triggered immunity

ROS:

Reactive oxygen species

SV:

Substomatal vesicle

TEM:

Transmission electron microscopy

WGA:

Wheat germ agglutinin

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Acknowledgments

This study has been financially supported by the National Key Basic Research Program of China (2013CB127700), Nature Science Foundation of China (No. 30571205; No. 30900931), 111 Project from the Ministry of Education of China (B07049), and talent-funded project of Northwest A&F University (No. 01140510).

Conflict of interest

All authors declare that they have no conflict of interest. We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “Subcellular localization of calcium in the incompatible and compatible interactions of wheat and Puccinia striiformis f. sp. tritici.”

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling, 712100, Shaanxi, China

    Shuining Yin, Chenfang Wang, Min Jiao, Feng Li, Qingmei Han, Lili Huang, Hongchang Zhang & Zhensheng Kang

  2. College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Shuining Yin, Chenfang Wang, Min Jiao, Feng Li, Lili Huang & Zhensheng Kang

  3. College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, China

    Hongchang Zhang

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  1. Shuining Yin
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  2. Chenfang Wang
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  3. Min Jiao
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  4. Feng Li
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Correspondence to Hongchang Zhang or Zhensheng Kang.

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Yin, S., Wang, C., Jiao, M. et al. Subcellular localization of calcium in the incompatible and compatible interactions of wheat and Puccinia striiformis f. sp. tritici . Protoplasma 252, 103–116 (2015). https://doi.org/10.1007/s00709-014-0659-3

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