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Exogenous sodium nitroprusside and glutathione alleviate copper toxicity by reducing copper uptake and oxidative damage in rice (Oryza sativa L.) seedlings

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Abstract

Nitric oxide (NO) and glutathione (GSH) regulate a variety of physiological processes and stress responses; however, their involvement in mitigating Cu toxicity in plants has not been extensively studied. This study investigated the interactive effect of exogenous sodium nitroprusside (SNP) and GSH on Cu homeostasis and Cu-induced oxidative damage in rice seedlings. Hydroponically grown 12-day-old seedlings were subjected to 100 μM CuSO4 alone and in combination with 200 μM SNP (an NO donor) and 200 μM GSH. Cu exposure for 48 h resulted in toxicity symptoms such as stunted growth, chlorosis, and rolling in leaves. Cu toxicity was also manifested by a sharp increase in lipoxygenase (LOX) activity, lipid peroxidation (MDA), hydrogen peroxide (H2O2), proline (Pro) content, and rapid reductions in biomass, chlorophyll (Chl), and relative water content (RWC). Cu-caused oxidative stress was evident by overaccumulation of reactive oxygen species (ROS; superoxide (O2 •–) and H2O2). Ascorbate (AsA) content decreased while GSH and phytochelatin (PC) content increased significantly in Cu-stressed seedlings. Exogenous SNP, GSH, or SNP + GSH decreased toxicity symptoms and diminished a Cu-induced increase in LOX activity, O2 •–, H2O2, MDA, and Pro content. They also counteracted a Cu-induced increase in superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), and glyoxalase I and glyoxalase II activities, which paralleled changes in ROS and MDA levels. These seedlings also showed a significant increase in catalase (CAT), glutathione peroxidase (GPX), dehydroascorbate reductase (DHAR), glutathione S-transferase (GST) activities, and AsA and PC content compared with the seedlings stressed with Cu alone. Cu analysis revealed that SNP and GSH restricted the accumulation of Cu in the roots and leaves of Cu-stressed seedlings. Our results suggest that Cu exposure provoked an oxidative burden while reduced Cu uptake and modulating the antioxidant defense and glyoxalase systems by adding SNP and GSH play an important role in alleviating Cu toxicity. Furthermore, the protective action of GSH and SNP + GSH was more efficient than SNP alone.

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Acknowledgements

M. G. Mostofa thankfully acknowledges the financial support from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

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Authors and Affiliations

  1. Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki, Kagawa, 761-0795, Japan

    Mohammad Golam Mostofa & Masayuki Fujita

  2. Department of Biochemistry and Molecular Biology, Bangabandhu Shiekh Mujibur Rahman Agricultural University, Gazipur, 1706, Bangladesh

    Mohammad Golam Mostofa

  3. Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, 1000, Bangladesh

    Zeba Islam Seraj

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  1. Mohammad Golam Mostofa
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  2. Zeba Islam Seraj
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Correspondence to Masayuki Fujita.

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Supplementary material table T1 Effect of exogenous 0.2 mM sodium ferrocyanide (SF) and 0.2 mM sodium nitroprusside (SNP) on visual appearances in 0.1 mM copper (Cu) -treated rice seedlings. Supplementary material Fig. S1 Effect of exogenous SNP (A), GSH (B), and SNP+GSH (C) on visual appearances in Cu-treated rice seedlings. Supplementary material Fig. S2 Effect of exogenous 0.2 mM sodium ferrocyanide (SF) and 0.2 mM sodium nitroprusside (SNP) on visual appearances in 0.1 mM copper (Cu) -treated rice seedlings. (PDF 522 kb)

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Mostofa, M.G., Seraj, Z.I. & Fujita, M. Exogenous sodium nitroprusside and glutathione alleviate copper toxicity by reducing copper uptake and oxidative damage in rice (Oryza sativa L.) seedlings. Protoplasma 251, 1373–1386 (2014). https://doi.org/10.1007/s00709-014-0639-7

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