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Tanshinone IIA and astragaloside IV promote the migration of mesenchymal stem cells by up-regulation of CXCR4

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Abstract

Mesenchymal stem cells (MSCs) have a therapeutic potential to treat cardiovascular diseases. However, a significant barrier to MSC therapy is insufficient MSC engraftment in ischemic myocardium after systemic administration. Here, we investigated the modulatory effects of tanshinone IIA and astragaloside IV on the migration of MSCs and further defined the underlying mechanisms. CXCR4 expression in MSCs was determined by using flow cytometry, real-time PCR, and western blotting. The results showed that CXCR4 expression was significantly higher in tanshinone IIA- and astragaloside IV-stimulated MSCs than that of the control. MSC migration toward stromal cell-derived factor-1α (SDF-1α) was studied using a transwell system. MSCs treated with tanshinone IIA and astragaloside IV showed stronger migration than that of the control. Moreover, this enhanced migration ability was abrogated by a CXCR4 inhibitor. In a rat acute myocardial infarction model, MSCs stimulated with tanshinone IIA and astragaloside IV were stained with Dio and injected into model rats via the tail vein. Dio-labeled cells in myocardium sections were observed by fluorescence microscopy. Tanshinone IIA- and astragaloside IV-stimulated MSCs showed enhanced capacities to home to ischemic myocardium sites. In addition, there was no significant difference in the SDF-1α expression among groups. These data suggest that tanshinone IIA and astragaloside IV regulate MSC mobilization, at least partially via modulation of the CXCR4 expression.

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Acknowledgment

We thank Prof. Hua-Han for his academic assistance.

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Authors and Affiliations

  1. Department of Traditional Chinese Medicine, Xijing Hospital, Fourth Military Medical University, 17 West Changle Road, Xi’an, Shanxi Province, 710032, People’s Republic of China

    Juan Xie, Zongren Wang, Feng Li, Jing Ma, Jun Chen, Yayun Nan, Hui Yi & Wen Wang

  2. Department of Dermatology, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shanxi Province, 710038, People’s Republic of China

    Huan Wang

  3. Department of Orthopedics, Xi’an Traditional Chinese Medicine Hospital, Xi’an, Shanxi Province, 710001, People’s Republic of China

    Tiebing Song

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  1. Juan Xie
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  2. Huan Wang
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  5. Feng Li
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  7. Jun Chen
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  9. Hui Yi
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  10. Wen Wang
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Correspondence to Wen Wang.

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Handling Editor: Peter Nick

Juan Xie, Huan Wang, Tiebing Song, and Wen Wang contributed equally to this work.

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Xie, J., Wang, H., Song, T. et al. Tanshinone IIA and astragaloside IV promote the migration of mesenchymal stem cells by up-regulation of CXCR4. Protoplasma 250, 521–530 (2013). https://doi.org/10.1007/s00709-012-0435-1

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  • DOI: https://doi.org/10.1007/s00709-012-0435-1

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