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Effect of hydrophobicity on intercalative binding of some surfactant copper(II) complexes with tRNA

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Abstract

The binding interaction between tRNA and surfactant copper(II) complexes with imidazo[4,5-f][1, 10]phenanthroline, 6,7,8,9-tetrahydrodipyrido[3,2-a:2′,4′-c]phenazine, and benzo[1]dipyrido[3,2-a′:2′,3′-c]phenazine ligands has been studied using various physicochemical techniques. In the presence of tRNA, UV–Vis spectrum of surfactant complexes showed red shift of the absorption band along with significant hypochromicity indicating intercalation of surfactant complexes with tRNA. Competitive binding study with ethidium bromide shows that complexes exhibit the ability to displace the tRNA-bound EB indicating that these complexes bind to tRNA in strong competition with EB for the intercalative binding site. Observed changes in the circular dichroic spectra of tRNA in the presence of surfactant complexes support the strong binding of complexes with tRNA. CV and viscosity results also affirm this mode of binding. The results reveal that the extent of tRNA binding of the benzo[1]dipyrido[3,2-a′:2′,3′-c]phenazine complex was greater than that of the other complexes. Binding to tRNA appears to be mainly intercalative in nature.

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Acknowledgments

We are grateful to the UGC-SAP and DST-FIST programmes of the Department of Chemistry, Bharathidasan University, and UGC-RFSMS fellowship to one of the authors, K. Nagaraj, by Bharathidasan University. Financial assistance from the CSIR (Grant No. 01(2461)/11/EMR-II), DST (Grant No. SR/S1/IC-13/2009), and UGC (Grant No. 41-223/2012(SR)) sanctioned to S. Arunachalam are also gratefully acknowledged.

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Authors and Affiliations

  1. School of Chemistry, Bharathidasan University, Trichirappalli, 620024, Tamilnadu, India

    Karuppiah Nagaraj, Subramanian Sakthinathan & Sankaralingam Arunachalam

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  1. Karuppiah Nagaraj
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  2. Subramanian Sakthinathan
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  3. Sankaralingam Arunachalam
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Correspondence to Sankaralingam Arunachalam.

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Nagaraj, K., Sakthinathan, S. & Arunachalam, S. Effect of hydrophobicity on intercalative binding of some surfactant copper(II) complexes with tRNA. Monatsh Chem 145, 1897–1902 (2014). https://doi.org/10.1007/s00706-014-1267-9

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  • DOI: https://doi.org/10.1007/s00706-014-1267-9

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