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Complete genome sequence and analysis of blackcurrant leaf chlorosis associated virus, a new member of the genus Idaeovirus

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Abstract

Blackcurrant leaf chlorosis associated virus (BCLCaV) was isolated from symptomatic blackcurrants (Ribes nigrum cv. Baldwin). The virus has a genome organization similar to that of raspberry bushy dwarf virus (RBDV), the type member of the genus Idaeovirus. The RNA-1of this virus encodes the replicase complex (ORF1, Mr 197 kDa), while RNA-2 encodes a putative movement protein (ORF2a, Mr 38.8 kDa) and the putative coat protein (ORF2b, Mr 30 kDa). A concatenated form of BCLCaV RNA-2 was detected by next-generation sequencing and confirmed by RT-PCR. BCLCaV is a new member of the genus Idaeovirus.

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Acknowledgements

This research was conducted with funding from the Canadian Food Inspection Agency’s Mandated Genomics and Research Development Initiative, Federal Government of Canada. We would like to thank the Diagnostic Section, Sidney Laboratory, Canadian Food Inspection Agency, for kindly providing information and support with heat therapy, disease testing, and the disease status of the blackcurrant (Ribes nigrum) accessions.

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  1. Sidney Laboratory, Centre for Plant Health, Canadian Food Inspection Agency, 8801 East Saanich Road, North Saanich, BC, V8L 1H3, Canada

    Delano James & James Phelan

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  1. Delano James
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  2. James Phelan
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Correspondence to Delano James.

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James, D., Phelan, J. Complete genome sequence and analysis of blackcurrant leaf chlorosis associated virus, a new member of the genus Idaeovirus . Arch Virol 162, 1705–1709 (2017). https://doi.org/10.1007/s00705-017-3257-9

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  • DOI: https://doi.org/10.1007/s00705-017-3257-9

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