Abstract
Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in to the poorly transmissible T36-based infectious clone. We found that introducing p65 or p61 sequences from FS577 significantly increased transmission efficacy. Interestingly, replacement of both genes produced a greater increase than either gene alone, suggesting that CTV transmission requires the concerted action of co-evolved p65 and p61 proteins.
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Acknowledgments
The authors wish to acknowledge the assistance of C. Robertson and R. McCoy for virus propagation, E. Carter for sampling of plants, and C.T. Bierman for performing ELISA assays. This research was supported by grants from Southern Gardens Citrus, by an endowment from the J. R. and Addie S. Graves family, and the UF Agricultural Experiment Station.
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The authors declare no conflict of interest. No human or animal subjects were used during this research.
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Harper, S.J., Killiny, N., Tatineni, S. et al. Sequence variation in two genes determines the efficacy of transmission of citrus tristeza virus by the brown citrus aphid. Arch Virol 161, 3555–3559 (2016). https://doi.org/10.1007/s00705-016-3070-x
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DOI: https://doi.org/10.1007/s00705-016-3070-x