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Genomic and proteomic characterization of SE-I, a temperate bacteriophage infecting Erysipelothrix rhusiopathiae

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Abstract

A bacteriophage infecting pathogenic Erysipelothrix rhusiopathiae was isolated from a swine farm experiencing an outbreak of acute swine erysipelas; we designated this phage SE-I. SE-I has an icosahedral head, a long tail and a double-stranded DNA genome. The 34,997-bp genome has a GC content of 34 % and contains 43 open reading frames (ORFs) encoding packaging, structural, lysin-holin, and hypothetical proteins. Components of purified SE-I were separated using SDS-PAGE and analyzed using liquid chromatography–mass spectrometry. Nine proteins were identified, encoded by ORF9, ORF15, ORF23, ORF30, ORF31, ORF33, ORF39, ORF40 and ORF 42. A phylogenetic tree constructed based on the sequence of the large terminase subunit revealed that SE-I is closely related to Staphylococcus phages P954 and phi3396. The CHAP-domain-containing protein encoded by ORF25 was expressed in E. coli and which was able to inactivate host bacteria. SE-I was able to infect 7 of 13 E. rhusiopathiae strains, but was unable to infect Salmonella, Streptococcus suis, and Staphylococcus aureus. This is the first report of the isolation, characterization, and genomic and proteomic analysis of a temperate phage infecting E. rhusiopathiae, and it might lead to the development of new anti- E. rhusiopathiae agents.

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Acknowledgments

This study was supported by public welfare Grants from the Ministry of Agriculture, the People’s Republic of China (201203039), and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). The authors thank Dr. Tanja Opriessnig (Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA) for offering to test serovars of the E. rhusiopathiae isolates.

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  1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China

    Wentao Yuan, Yaning Zhang, Guangcao Wang, Juan Bai, Xianwei Wang, Yufeng Li & Ping Jiang

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Correspondence to Yufeng Li.

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Yuan, W., Zhang, Y., Wang, G. et al. Genomic and proteomic characterization of SE-I, a temperate bacteriophage infecting Erysipelothrix rhusiopathiae . Arch Virol 161, 3137–3150 (2016). https://doi.org/10.1007/s00705-016-3018-1

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