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Hepatitis B virus resistance substitutions: long-term analysis by next-generation sequencing

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Abstract

HBV phylogenetics and resistance-associated mutations (RAMs) were surveyed by next-generation sequencing of 21 longitudinal samples from seven patients entering antiviral therapy. The virus populations were dominated by a few abundant lineages that coexisted with substantial numbers of low-frequency variants. A few low-frequency RAMs were observed before treatment, but new ones emerged, and their frequencies increased during therapy. Together, these results support the idea that chronic HBV infection is dominated by a few virus lineages and that an accompanying plethora of diverse, low-frequency variants may function as a reservoir that potentially contribute to viral genetic plasticity, potentially affecting patient outcome.

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Correspondence to Jorge Quarleri.

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Funding

This work was supported by the Argentinean National Agency of Scientific and Technological Promotion (grant number PICT 2012-0422 to JQ and PICT PRH-14 120 to LRJ); the University of Buenos Aires (grant number 20020110100034); and the Argentinean National Scientific and Technical Research Council (grant number PIP-11220110101089).

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the institution and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study was approved by the Huesped Foundation Ethics Committee, Buenos Aires, Argentina, and informed consent was obtained from each patient.

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Informed consent was obtained from all individual participants included in the study.

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Jones, L.R., Sede, M., Manrique, J.M. et al. Hepatitis B virus resistance substitutions: long-term analysis by next-generation sequencing. Arch Virol 161, 2885–2891 (2016). https://doi.org/10.1007/s00705-016-2959-8

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  • DOI: https://doi.org/10.1007/s00705-016-2959-8

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