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Eukaryotic elongation factor 1-beta interacts with the 5′ untranslated region of the M gene of Nipah virus to promote mRNA translation

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Abstract

Nipah virus belongs to the genus Henipavirus in the family Paramyxoviridae, and its RNA genome is larger than those of other paramyxoviruses because it has long untranslated regions (UTRs) in each gene. However, the functions of these UTRs are not fully understood. In this study, we investigated the functions of the 5′ UTRs and found that the 5′ UTR of the M gene upregulated the translation of a reporter gene. Using an RNA pull-down assay, we showed that eukaryotic elongation factor 1-beta (EEF1B2) interacts with nucleotides 81–100 of the M 5′ UTR and specifically enhances its translation efficiency. Our results suggest that the M 5′ UTR promotes the production of M protein and viral budding by recruiting EEF1B2.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research (KAKENHI, grant number 20790351 and 22228005) from the Japan Society for the Promotion of Science (JSPS). The mass-spectrometric analysis was conducted by the Laboratory Center for Proteomics Research (Institution of Medical Science, The University of Tokyo).

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Authors and Affiliations

  1. Laboratory Animal Research Center, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639, Japan

    Shotaro Uchida, Hiroki Sato, Misako Yoneda & Chieko Kai

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  1. Shotaro Uchida
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  2. Hiroki Sato
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  3. Misako Yoneda
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  4. Chieko Kai
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Correspondence to Chieko Kai.

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Uchida, S., Sato, H., Yoneda, M. et al. Eukaryotic elongation factor 1-beta interacts with the 5′ untranslated region of the M gene of Nipah virus to promote mRNA translation. Arch Virol 161, 2361–2368 (2016). https://doi.org/10.1007/s00705-016-2903-y

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  • DOI: https://doi.org/10.1007/s00705-016-2903-y

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