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The complete nucleotide sequence and genomic characterization of tropical soda apple mosaic virus

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Abstract

We report the first complete genome sequence of tropical soda apple mosaic virus (TSAMV), a tobamovirus originally isolated from tropical soda apple (Solanum viarum) collected in Okeechobee, Florida. The complete genome of TSAMV is 6,350 nucleotides long and contains four open reading frames encoding the following proteins: i) 126-kDa methyltransferase/helicase (3354 nt), ii) 183-kDa polymerase (4839 nt), iii) movement protein (771 nt) and iv) coat protein (483 nt). The complete genome sequence of TSAMV shares 80.4 % nucleotide sequence identity with pepper mild mottle virus (PMMoV) and 71.2-74.2 % identity with other tobamoviruses naturally infecting members of the Solanaceae plant family. Phylogenetic analysis of the deduced amino acid sequences of the 126-kDa and 183-kDa proteins and the complete genome sequence place TSAMV in a subcluster with PMMoV within the Solanaceae-infecting subgroup of tobamoviruses.

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Acknowledgments

We thank Carrie Vanderspool, Megan Carroll and Yaowapa Tantiwanich for their excellent technical assistance. Financial support was provided in part by The Banack Family Partnership Endowed Teaching Chairs in Agriculture to P.P. and T.D.

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Authors and Affiliations

  1. Biology Department, Indian River State College, 3209 Virginia Ave., Fort Pierce, FL, 34981, USA

    Kornelia Fillmer, Patchara Pongam & Tom D’Elia

  2. USDA, ARS, US Horticultural Research Laboratory, 2001 South Rock Rd, Fort Pierce, FL, 34945, USA

    Scott Adkins

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  1. Kornelia Fillmer
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  2. Scott Adkins
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  3. Patchara Pongam
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  4. Tom D’Elia
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Correspondence to Scott Adkins or Tom D’Elia.

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Fillmer, K., Adkins, S., Pongam, P. et al. The complete nucleotide sequence and genomic characterization of tropical soda apple mosaic virus. Arch Virol 161, 2317–2320 (2016). https://doi.org/10.1007/s00705-016-2888-6

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  • DOI: https://doi.org/10.1007/s00705-016-2888-6

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