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Screening of human bocavirus in surgically excised cancer specimens

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Abstract

Human bocavirus (HBoV) is a prevalent virus worldwide and is mainly associated with respiratory disorders. Recently, it was detected in several disease conditions, including cancers. Colorectal cancer (CRC) is the third main cause of cancers worldwide. Risk factors that initiate cell transformation include nutritional, hereditary and infectious causes. The aim of the current study was to screen for the presence of HBoV in solid tumors of colorectal cancer and to determine the genotypes of the detected strains. Surgically excised and paraffin-embedded colorectal cancer tissue specimens from 101 male and female patients with and without metastasis were collected over the last four years. Pathological analysis and tumor stages were determined. The presence of HBoV was screened by polymerase chain reaction, and the genotype of the detected HBoV was determined by direct gene sequencing. Most of the examined specimens were adenocarcinoma with mucinous activity in many of them. Twenty-four out of 101 (23.8 %) CRC tissue specimens were found to contain HBoV-1. Low sequence diversity was recorded in the detected strains. The virus was detected in both male and female patients with an age range of 30-75 years. It is proposed that HBoV-1 could play a potential role in the induction of CRC.

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Acknowledgments

This study was supported by the Taif University, through the Grant project number (3970-436-1).

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Authors and Affiliations

  1. College of Medicine, Taif University, Al-Taif, Saudi Arabia

    Ahmed S. Abdel-Moneim & Taha Z. M. Mahran

  2. Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt

    Ahmed S. Abdel-Moneim

  3. Department of Surgical Oncology, Faculty of Medicine, Menofia University, Monufia, Egypt

    Hosam A. El-Fol & Ahmed S. El-Gammal

  4. Department of Clinical Pathology National Cancer Institute, Cairo University, Cairo, Egypt

    Mahmoud M. Kamel

  5. Department of Pathology, National Research Institute, Cairo University, Cairo, Egypt

    Ahmed S. A. Soliman

  6. Department of Biology, College of Science, Taif University, Al-Taif, Saudi Arabia

    Emad A. Mahdi

  7. Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt

    Emad A. Mahdi

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  1. Ahmed S. Abdel-Moneim
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Correspondence to Ahmed S. Abdel-Moneim.

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705_2016_2885_MOESM1_ESM.tif

SUPPL. 1 Deduced amino acid sequence of the terminal VP1 protein motifs of different HBoV-1 strains detected in CRC cases. a Amino acid numbering based on ST1 (accession number DQ000495) VP1 (TIFF 195 kb)

Supplementary material 2 (DOC 72 kb)

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Abdel-Moneim, A.S., El-Fol, H.A., Kamel, M.M. et al. Screening of human bocavirus in surgically excised cancer specimens. Arch Virol 161, 2095–2102 (2016). https://doi.org/10.1007/s00705-016-2885-9

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  • DOI: https://doi.org/10.1007/s00705-016-2885-9

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