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Detection and molecular characterization of a novel piscine-myocarditis-like virus from baitfish in the USA

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Abstract

During a survey of apparently healthy baitfish from the state of Minnesota, a novel piscine-myocarditis-like virus (PMCLV) was detected in golden shiners (Notemigonus crysoleucas). The nearly complete genome sequence is 5819 nt long, including a partial 5’ untranslated region (UTR) of 100 nt. The sequence is divided into three ORFs: the complete ORF1 and ORF2, encoding proteins of 818 and 831 amino acids, respectively, and a partial ORF3 encoding 248 amino acids of the corresponding protein. This novel virus sequence was most closely related to piscine myocarditis virus (PMCV), with a 49.0 % and 58.2 % amino acid identity in the ORF1 (YP005481249)- and ORF2 (YP004581250)-encoded proteins, respectively. Six of 56 retail outlets (e.g., bait shops) were positive during the 2014–2015 survey, indicating a 10.7 % prevalence of the novel virus in this population of golden shiners. Currently, there is no disease that is known to be associated with this virus.

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Acknowledgments

The authors thank the University of Minnesota Veterinary Diagnostic Laboratory.

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Correspondence to Sunil K. Mor.

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Mor, S.K., Phelps, N.B.D. Detection and molecular characterization of a novel piscine-myocarditis-like virus from baitfish in the USA. Arch Virol 161, 1925–1931 (2016). https://doi.org/10.1007/s00705-016-2873-0

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  • DOI: https://doi.org/10.1007/s00705-016-2873-0

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