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A new genotype of border disease virus with implications for molecular diagnostics

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Abstract

Border disease virus (BDV) is a (+) single-stranded RNA pestivirus affecting mainly sheep and goats worldwide. Genetic typing of BDV has led to the identification of at least seven major genotypes. This study reports the detection of a BDV strain from a goat in northwestern Italy during routine investigations. Sequence analysis revealed mutations in the 5’-UTR of the virus with implications for BDV molecular diagnostics. Moreover, subsequent phylogenetic analysis based on the combined 5’-UTR and Npro/partial C genes, showed divergence from known BDV genotypes, revealing the detection of a novel pestivirus group, for which we propose the name BDV genotype 8.

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Acknowledgments

Funding for the work was provided by the Italian Ministry of Health (grant IZS PLV 16/11 RC). We thank Dr. Gian Mario De Mia and Dr. Monica Giammarioli (National Reference Center for Pestivirus, Perugia, Italy) for providing protocols for Npro amplification.

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Authors and Affiliations

  1. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, via Bologna 148, 10154, Turin (TO), Italy

    Simone Peletto, Claudio Caruso, Francesco Cerutti, Paola Modesto, Simona Zoppi, Alessandro Dondo, Pier Luigi Acutis & Loretta Masoero

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  1. Simone Peletto
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  2. Claudio Caruso
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  3. Francesco Cerutti
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  4. Paola Modesto
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  5. Simona Zoppi
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  6. Alessandro Dondo
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  7. Pier Luigi Acutis
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  8. Loretta Masoero
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Correspondence to Simone Peletto.

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705_2015_2696_MOESM1_ESM.xls

Additional file 1. Divergence and percent identity for pairs of 5’-UTR sequences among ruminant pestiviruses calculated using the MegAlign software (DNASTAR Inc.). The dataset includes the newly detected BDV-8 isolate and other pestivirus reference sequences enriched for representatives of BDV major genotypes (XLS 26 kb)

705_2015_2696_MOESM2_ESM.xls

Additional file 2. Divergence and percent identity for pairs of Npro sequences among ruminant pestiviruses calculated using the MegAlign software (DNASTAR Inc.). The dataset includes the newly detected BDV-8 isolate and other pestivirus reference sequences enriched for representatives of BDV major genotypes (XLS 31 kb)

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Peletto, S., Caruso, C., Cerutti, F. et al. A new genotype of border disease virus with implications for molecular diagnostics. Arch Virol 161, 471–477 (2016). https://doi.org/10.1007/s00705-015-2696-4

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  • DOI: https://doi.org/10.1007/s00705-015-2696-4

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